垂体腺瘤E-钙粘素和β-连环素的表达及意义  

Expression and significance of E-cadherin and β-catenins in pituitary adenoma

作　　者：周开宇[1] 王广涛[1] 罗永康[1] 金杭煌[1] 

机构地区：[1]台州学院医学院附属台州市立医院神经外科,浙江台州318000

出　　处：《中国卫生检验杂志》2009年第12期2881-2883,共3页Chinese Journal of Health Laboratory Technology

基　　金：浙江省台州学院基金资助项目(08ND33);浙江省台州市科技局基金资助项目(051KY48);浙江省台州市椒江区科技局基金资助项目(31067)

摘　　要：目的:采用免疫组化方法检测人类垂体腺瘤中E-钙粘素和β-连环素的表达,为进一步了解人类垂体腺瘤的生物学行为提供依据。方法:对41例垂体腺瘤病理标本采用免疫组化EnVision法,以单克隆抗体直接检测E-钙粘素、β-连环素的表达。按染色强度和阳性范围评估免疫组化结果,分析粘附分子表达程度与垂体腺瘤的病理亚型、肿瘤侵袭性的相关关系。结果:泌乳素型和生长激素型间,E-钙粘素、β-连环素的阳性表达程度差别均有统计学意义(uc=2.00、2.08,P<0.05);侵袭性、非侵袭性垂体腺瘤间,E-钙粘素、β-连环素的阳性表达程度差别均有统计学意义(uc=2.13、1.98,P<0.05)。结论:E-钙粘素、β-连环素表达与垂体腺瘤的不同病理亚型相关,在生长激素型垂体腺瘤中,存在着E-钙粘素、β-连环素表达明显下调现象;垂体腺瘤侵袭性与E-钙粘素、β-连环素的低表达相关。Objective：To analyze the expression of E-cadherin and β-catenin in human pituitary adenoma by immunohistochemistry for further understanding of biological benaviors of pituitary adenoma.Methods：The specimens included in this study were collected form 41 pituitary adenoma cases,EnVision was used in immunohistochemical staining,The E-cadherin and β-catenin were direct tested by the monoclonal antibodies.The results were graded depending on the staining intensity and positive range of the immunoreactivity,the correlation between the expression of the E-cadherin and β-catenin with the subtype pituitary adenoma and the invasiveness of pituitary adenoma were analyzed.Results：In the prolactinomas and growth hormone-secreting pituitary adenoma,the positive staining degrees of the E-cadherin,β-catenin were insignificantly different（uc=2.00,2.08 respectively,P0.05;In the invasive and non-invasive pituitary adenoma,the positive staining degrees of the E-cadherin,β-catenin were insignificantly different（uc=2.13,1.98 respectively,P0.05）.Conclusion：The expression of E-cadherin and β-catenin in the pituitary adenoma is related to the subtype pituitary adenoma,the expression of E-cadherin and β-catenin in the growth hormone-secreting pituitary adenoma were remarkably decrease.The invasive pituitary adenoma is related to the expression of E-cadherin and β-catenin significantly down-regulated.

关 键 词：垂体腺瘤 E-钙粘素 Β-连环素 

分 类 号：R736.4[医药卫生—肿瘤]