拮抗放线菌S24的鉴定及其对黄曲霉的抑制作用  

Identification of Actinomycete Strain S24 and Its Antagonistic Effects on Aspergillus flavus

作　　者：周启升[1] 孙长坡[2] 张楠[3] 仇念全[1] 曹宁宁[1] 张本峰[1] 刘训理[1] 

机构地区：[1]山东农业大学林学院,山东泰安271018 [2]国家粮食局科学研究院,北京100037 [3]山东农业大学生命科学学院,山东泰安271018

出　　处：《微生物学通报》2009年第12期1832-1837,共6页Microbiology China

摘　　要：以黄曲霉(Aspergillus flavus)为靶标,从泰山土壤中分离获得一株对黄曲霉、赭曲霉、黑曲霉等粮食和饲料中常见的曲霉菌有高效拮抗活性的放线菌S24。根据其形态特征、培养特征、理化性质、细胞壁组份及16S rRNA序列分析,初步判定该菌株为链霉菌属中的白网链霉菌(Streptomyces albireticuli)的近似种;该菌株抗菌谱广,胞外抗菌物质对热稳定,100°C加热100min抗菌活性无明显变化;96孔板法测得其胞外抗菌物质粗提物对黄曲霉的最小抑/杀菌浓度(MIC/MFC)分别为19.53μg/mL和39.06μg/mL。Taking Aspergillusflavus as the target, Actinomyces S24 isolated from Mountain Tai soil showed strong antagonistic activity against Aspergillus flavus, Aspergillus niger, Asperegillus alutacells and the other common Aspergillus in food and feed. Based on its morphologic, culture, physiological and biochemi- cal characteristics, chemotaxonorny as well as 16S rRNA sequence analysis, strain S24 was placed within the genus Streptomyces and identified as a similar species with Streptomyces albireticuli, which had a broad inhibition spectrum and its fermentation characteristics were outstanding. The antibacterial substance was stable. Deposed for 100 min at 100℃, there was no obvious influence on its antibacterial activity. Assay using 96-well plate illustrated that the minimum inhibitor concentration （MIC） and minimum antifungal concentration （MFC） of extracellular crude antibacterial extracts against A. flavus were 19.53 μg/mL and 39.06μg/mL, respectively.

关 键 词：黄曲霉 白网链霉菌 鉴定 最小抑/杀菌浓度 

分 类 号：S476[农业科学—农业昆虫与害虫防治]