以壳聚糖为载体固定化谷氨酰胺转氨酶的研究  

To chitosan as a carrier immobilized study glutamine transaminase

作　　者：张春红[1] 高慧楠[1] 常南[1] 郭丽嘉[1] 

机构地区：[1]沈阳农业大学食品学院,沈阳110161

出　　处：《食品科技》2009年第12期33-35,共3页Food Science and Technology

基　　金：辽宁省科技厅科技攻关项目(2004205001);辽宁省教育厅项目(2005369)

摘　　要：研究利用壳聚糖固定化谷氨酰胺转胺酶的最佳条件,并对固定化酶的性质进行了探讨。结果表明:当戊二醛浓度为2.5%、酶浓度为10%、pH7.0、25℃下固定化1.5h,酶活回收率为49.7%,固定化酶的pH值稳定性、热稳定性、操作稳定性及贮存稳定性都比游离酶显著提高。This paper studies the use of chitosan immobilized transglutaminase the best conditions,and the nature of immobilized enzyme are discussed. The results showed that：when glutaraldehyde concentration was 2.5% ,enzyme concentration of 10% ,pH7.0,25 ℃ under immobilized 1.5 h,activity recovery rate of 49.7 percent. The pH value of immobilized enzyme stability,thermal stability,operational stability and storage stability than the free enzyme markedly improved.

关 键 词：谷氨酰胺转氨酶 壳聚糖 戊二醛 固定化 

分 类 号：TQ814.7[化学工程]