色氨酸-镝(Ⅲ)配合物与鲱鱼精DNA的作用方式  

Binding Mode between Trp-Dy(Ⅲ) Complex and Herring Sperm DNA

作　　者：赵娜[1] 王兴明[1] 王单[1] 徐妍[1] 潘海专[1] 丁立生[2] 

机构地区：[1]西南科技大学材料科学与工程学院,四川绵阳621010 [2]中国科学院成都生物研究所,四川成都610041

出　　处：《无机化学学报》2009年第12期2129-2136,共8页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金资助项目(No.30572254);西南科技大学研究生创新基金资助项目

摘　　要：采用UV光谱法、荧光光谱法,在pH=7.40的缓冲溶液中确定了镝髥与色氨酸的结合比nDy髥∶nTrp=1∶3,Dy髥(Trp)3配合物与鲱鱼精DNA的结合比nDy髥(Trp)3∶nDNA=2∶1。用双倒数法确定了结合常数K25℃=5.75×104L·mol-1和K37℃=3.27×104L·mol-1。化学热力学研究显示配合物Dy髥(Trp)3与hsDNA的结合为熵和焓共同驱动。结合Scatchard法和粘度法,确定了配合物Dy髥(Trp)3与hsDNA之间主要为静电作用和嵌插作用。By means of UV and fluorescence spectra, the binding ratios between Dy（Ⅲ）（Trp）3 and DNA. in physiological pH environment（pH7.40） were determined as nDy（Ⅲ）：nDNA=2：1 and nDy（Ⅲ）：nTrp=1：3. The binding constant of K25℃=5.75×10^4L·mol^-1 and K37℃=5.75×10^5L·mol^-1 were obtained by double reciprocal method. Thermodynamic function computation demonstrates that △rS⊙m and △rH⊙m are the primary driving power of the interaction between Dy（Ⅲ）（Trp）3 and DNA. By combination analysis of the Scatchard and viscometry methods, we suggested that the interaction mode between Dy（Ⅲ）（Trp）3 complex and herring sperm DNA be electrostatic and intercalation bindings.

关 键 词：色氨酸 镝髥 鲱鱼精DNA 作用方式 

分 类 号：O614.342[理学—无机化学]