关节腔注射TGF-1治疗兔颞下颌关节骨关节炎的实验研究  

Treatment of temporomandibular joint osteoarthritis by injection of transforming growth factor-beta1 in rabbit model

作　　者：满城[1,2] 张碧[1] 马永清[1] 胡静[3] 祝颂松[3] 

机构地区：[1]四川大学华西口腔医学院口腔疾病研究国家重点实验室,四川成都610041 [2]遵义医学院附属口腔医院口腔颌面外科,贵州遵义563003 [3]四川大学华西口腔医院口腔颌面外科,四川成都610041

出　　处：《临床口腔医学杂志》2009年第12期714-717,共4页Journal of Clinical Stomatology

基　　金：国家自然科基金资助(30700951);教育部博士点青年教师基金资助(JS20071226506549)

摘　　要：目的:探讨关节腔注射TGF-1治疗兔颞下颌关节骨关节炎(TMJOA)的效果。方法:24只新西兰大白兔采用双侧关节盘部分切除方法建立TMJOA动物模型,于建模后4周于右侧(实验侧)TMJ关节腔一次性注射TGF-150μl,左侧(对照侧)注射等量生理盐水。关节腔注射后12周、24周分别处死12只动物,解剖颞下颌关节,进行组织学及RT-PCR检测。结果:所有关节均表现出骨关节炎病理改变,术后12周、24周实验组病损较对照组轻,RT-PCR检测结果提示术后12周,实验组II型胶原及蛋白聚糖表达升高,且较对照组强(P<0.05),IL-1及蛋白聚糖酶表达与对照组无明显差异(P>0.05);术后24周实验组所有检测指标与对照组无明显差异(P>0.05)。结论:重组TGF-1因子关节腔注射能阻止TMJOA关节软骨的破坏,对TMJOA产生治疗作用。Objective：To investigate the effects of transforming growth factor-1 （TGF-1） in temporomandibular joint osteoarthritis through intra-articular injection. Method：Disc perforation was performed bilaterally in 24 rabbits to induce osteoarthritis （OA）. The right joint was injected with TGF-β as experimental groups,and the left joint was physiological saline as controls. At 12 and 24 weeks after injection, 12 animals were sacrificed and the TMJ was dissected, was performed for comparison of histology and RT-PCR. Result：All joints showed osteoarthritic-like changes, but the degree in the experimental was significantly less severe than the control. At 12 weeks a significantly greater expression of aggrecan and collagen type Ⅱ was found in the experimental when compared to controls. How- ever, no difference in either anabolic or catabolic genes was found between two groups at 24 weeks.Conclusion： TGF-β may have a potential benefit in protecting articular cartilage during the development of TMJOA.

关 键 词：颞下颌关节 骨关节炎 转化生长因子-1 治疗 

分 类 号：R782.6[医药卫生—口腔医学]