适合棉花品种鉴定的SSR核心引物的筛选  

Screening of SSR Core Primer Pairs for Identificating Cotton Cultivar

作　　者：刘峰[1] 冯雪梅[1] 钟文[2] 刘玉栋[1] 阴祖军[1] 韩秀兰[1] 徐子初 沈法富[1] 

机构地区：[1]作物生物学国家重点实验室,山东农业大学农学院,泰安271018 [2]山东省种子管理总站,济南250100 [3]山东鑫秋种业科技有限公司,德州253200

出　　处：《分子植物育种》2009年第6期1160-1168,共9页Molecular Plant Breeding

摘　　要：本研究利用来自Cotton Marker Database(CMD)数据库中的2000个引物对32份审定棉花品种基因组DNA进行了PCR扩增,筛选出26个多态性核心引物,其中11个引物被推荐为棉花品种鉴定的首选核心引物,同时提出了核心引物理论上可区分的最大品种数的计算公式:N=G1×…×Gn,理论推测结果表明:利用11个首选核心引物进行棉花品种鉴定是可行的。建立在SSR指纹数据基础上的不同组合品种(系)间聚类分析以及对25份区试品系和A品种的真实性鉴定,结果表明:所有参试品种(系)遗传基础狭窄,32份审定品种间DNA水平上的差异与品种来源的系谱分析结果基本吻合,首选核心引物不仅可以对品种(系)的真实性做出鉴定,而且对遗传距离狭窄的品种(系)具有很好的鉴别力。In this study,genomic DNA from thirty-two approved cotton cultivars were amplified with 2 000 primer pairs selecting from cotton marker database （CMD） by using polymerase chain reaction （PCR）,twenty-six core primer pairs with polymorphism were selected,of which eleven core primer pairs were proposed as preferred primer pairs for identification of cotton cultivars,at the same time a formula for calculating the maximum number of cotton cultivars that the eleven core primer pairs can identify in theory was advanced：N=G1×...×Gn,and according to theory,it has deduced that eleven core primer pairs were effective.Genetic clustering analysis of different com-binations of cotton cutlivars （lines） used in this study and authenticity identification of A approved cutlivar and twentyfive cotton varieties in Shandong provincial regional trial were performed based on SSRs fingerprint database,the result showed that genetic basis of the tested materials were limited,difference of thirtytwo approved cotton cultivars at DNA level was in accordance with the result of pedigree analysis of them,the preferred eleven core prime pairs not only can identify the authenticity of cotton cultivars （lines）,but also had good power in identification of cotton cultivars （lines） with limited genetic basis.

关 键 词：棉花 SSR 品种鉴定 核心引物 

分 类 号：S562[农业科学—作物学]