马铃薯耐热无性系离体筛选的初步研究  

Preliminary Studies on in Vitro Selection for Potato Clonal Lines with Heat-Tolerance

作　　者：宁志怨[1] 董玲[1] 陈静娴[1] 廖华俊[1] 江芹[1] 

机构地区：[1]安徽农业科学院园艺研究所,合肥20031

出　　处：《分子植物育种》2009年第6期1176-1179,共4页Molecular Plant Breeding

基　　金：安徽省农业科学院院长青年基金项目资助

摘　　要：为了获得耐热性较高的马铃薯品种,本研究以马铃薯试管苗的愈伤组织为供试材料,采用羟脯氨酸(HYP)和热胁迫相结合的方法来进行马铃薯耐热无性系的离体筛选研究。研究结果表明,MS+1.5～3.0mg/L6-BA+0.1mg/LNAA的培养基均能够诱导全部供试马铃薯茎段产生愈伤组织,说明细胞分裂素6-BA对愈伤组织的形成有着非常重要的作用;同时确定了马铃薯愈伤组织耐羟脯氨酸筛选的适宜浓度为0.2～0.4mg/mL。MS+1.0～5.0mg/LBA+0.1mg/LNAA+5mg/LGA的分化培养基能够诱导愈伤组织进行植株再生,但分化率较低,只有8%～25%。结果表明不同浓度的分化培养基均能诱导马铃薯愈伤组织分化成苗,本实验还筛选出热胁迫的适宜条件为42℃24h,且经过羟脯氨酸与高温胁迫结合多次筛选获得了两个耐热无性系NKY1和NKY2。初步表明本筛选方法可以用于马铃薯耐热无性系的筛选。In order to obtain the heat-tolerance potato cultivars,we employed the potato test-tube plantlet callus as materials for the selection of heat-tolerant clonal lines of potato in vitro by using the combinative method of Hydrox-yproline-resisitant and high temperature stress in this research.The results showed that all the tested potato stem segments can be induced to generate callus by the medium MS＋1.5-3.0 mg/L 6-BA＋0.1 mg/L NAA,which illustrat-ed that the cytokinin 6-BA played an very important part in the callus formation.Meanwhile,we have also found that the optimal concentration of HYP for selection is 0.2-0.4 mg/mL.The differential medium MS＋1.0-5.0 mg/L BA＋0.1 mg/L NAA＋5 mg/L GA can induce the callus for plantlet regeneration,but the differentiation rate was comparatively low,only between 8% and 25%.Experiments revealed that differential medium of different concentrations can induce the patato callus differentiate into seedling.We have determined that the optimal condition of high temperature stress selection was 42℃ for 24 hours.After repeated tests,we acquired two heat-tolerant potato clonal lines,designated as NKY1 and NKY2.Preliminary data show that this kind of selection approach is suitable for the selection of heat-tolerant potato clonal lines.

关 键 词：马铃薯 耐热无性系 离体筛选 热胁迫 

分 类 号：S532[农业科学—作物学] S562