CTGF作用于体外培养牛晶状体上皮细胞的研究  

The effects of connective tissue growth factor on bovine lens epithelial cells in vitro

作　　者：陈博[1] 曹阳[1] 胡义珍[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院眼科,武汉430022

出　　处：《眼科研究》2009年第12期1068-1072,共5页Chinese Ophthalmic Research

基　　金：湖北省自然科学基金资助(2006ABA097)

摘　　要：目的观察结缔组织生长因子(CTGF)在体外培养牛晶状体上皮细胞(LECs)迁移和转分化中的作用。方法将体外培养的第2～3代牛LECs分为对照组和实验组,实验组分别经0.1×106、0.5×106、1.0×106ng/LCTGF处理24h后,采用半定量RT-PCR和Westernblot技术检测α-平滑肌肌动蛋白(α-SMA)mRNA以及蛋白表达的变化。迁移实验中,利用transwell小室对LECs的移行能力进行检测。结果与空白对照组相比,CTGF能够明显促进α-SMAmRNA以及蛋白的表达(F=66.56,P<0.01;F=65.43,P<0.01)。迁移实验发现,CTGF能够促进LECs的移行,与空白对照组相比差异有统计学意义(t=51.7,P<0.01)。结论CTGF以剂量依赖的方式促进牛LECs的分化和迁移,可能在后囊膜混浊(PCO)的LECs移行、转分化以及细胞外基质沉积的过程中发挥重要作用。Objective This study was to observe the effects of connective tissue growth factor（CTGF）on migration and transdifferentiation of bovine lens epithelial cells（BLECs）.MethodsThe culture and identification of BLECs adopted the method of Hu（reference 1）.The 2-3 passages of BLECs were collected and used in this experiment at the concentration of 1×106 cells/hole.The free-serum DMEM containing 0.1ng/L,0.5ng/L,1.0ng/L of CTGF was added into medium for 24 hours in different experimental group respectively,and only equal volume of free-serum DMEM was added in control group.Expression of α-smooth muscle actin（α-SMA）mRNA and protein in the BLECs were examined by semiquantitative RT-PCR and Western blot,respectively.The transwell inserts were used to evaluate the migration ability of BLECs.ResultsThe expression of α-SMA mRNA in cultrued BLECs was gradually increased in different concentrations of CTGF groups.Compared with control group,the expression of α-SMA mRNA in experimental group was significantly enhanced（F=66.56,P〈0.01）.The expression of α-SMA protein followed the same pattern（F=65.43,P〈0.01）.The migration ability of BLECs was obviously elevated after CTGF stimulation under the light microscope.The migration rate of BLECs was considerably increased in experimental group compared with blank control group（t=51.7,P〈0.01）.ConclusionCTGF promotes the migration and transdifferentiation of BLECs at a dose-dependent manner in vitro.CTGF plays an important role in the formation of posterior capsule opacification.

关 键 词：结缔组织生长因子 晶状体上皮细胞 迁移 平滑肌肌动蛋白 白内障 

分 类 号：R776.1[医药卫生—眼科]