体外培养人眼小梁细胞Ang Ⅱ的表达  

Expression of angiotensin Ⅱ in cultured human trabecular meshwork cell

作　　者：章艳[1] 熊新春[2] 郑红梅[2] 秦文[2] 

机构地区：[1]宜昌市第一人民医院(三峡大学人民医院)眼科,443000 [2]华中科技大学协和医院眼科,武汉430022

出　　处：《眼科研究》2009年第12期1077-1079,共3页Chinese Ophthalmic Research

基　　金：湖北省自然科学基金资助(2006ABA135)

摘　　要：目的探讨血管紧张素Ⅱ(Ang Ⅱ)在体外培养的人眼小梁细胞(TMC)中的表达情况。方法原代和传代培养人眼TMC株,收集第3代人眼TMC用于制作细胞爬片。利用免疫组织化学染色法和Western blot法检测体外培养人眼TMC中AngⅡ蛋白的表达。结果传代培养的人眼TMC呈典型梭形,免疫组织化学检测显示体外培养的人眼TMC细胞膜和细胞质中可见AngⅡ的阳性颗粒,阴形对照片中未见有Ang Ⅱ蛋白表达。Western blot法在相对分子质量为64000处可见一明显蛋白条带,为Ang Ⅱ蛋白的阳性表达。结论Ang Ⅱ在体外培养的人眼TMC中呈阳性表达,提示AngⅡ可能参与青光眼眼压和房水循环的调节。Objective Research showed that angiotensin converting enzyme inhibitor and angiotensinⅡ（AngⅡ）receptor antagonist has good role of lowing-intraocular pressure.This study was to explore whether cultured human trabecular meshwork cells express AngⅡin vitro.MethodsThe human trabecular meshwork cells strains were cultured in DMEM＋F12 medium containing 25% fetal bovine serum in vitro and passaged at the proportion of 1∶3.The third generation of cells were collected and cell climbing sheet was prepared.The expression of AngⅡ in human trabecular meshwork cells was examined by immunohistochemistry,and AngⅡ protein was localized by Western blot.ResultsSubcultured cells showed spindle shape.AngⅡwas positively expressed in human trabecular meshwork cells by immunochemistry,showing the yellow-brown granule in cellular membrane and cytoplasm.A absence of response for AngⅡwas found in negative control sample.The band of AngⅡ protein was found at the relative molecular weight of 64000 by Western blot.ConclusionThe result implies that human trabecular meshwork cells have the ability of synthesizing AngⅡ.It suggests that AngⅡ participates in the regulation of intraocular tension in glaucomous eye.

关 键 词：人眼小梁细胞 血管紧张素Ⅱ 眼压 

分 类 号：R775[医药卫生—眼科]