Ⅰ型和Ⅳ型胶原及纤维连接蛋白在喉鳞状细胞癌中的表达及其与肿瘤的临床关系  

Expression and pathobiological significance of ColⅠ,ColⅣ and Fn in laryngeal squamous cell carcinomas

作　　者：张海琴[1,2] 李薇[1] 尚伟[3] 

机构地区：[1]青岛大学医学院附属医院耳鼻咽喉头颈外科,山东青岛266003 [2]杭州师范大学附属医院耳鼻咽喉科,杭州310000 [3]青岛大学医学院附属医院口腔颌面外科

出　　处：《临床耳鼻咽喉头颈外科杂志》2009年第24期1130-1133,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：山东省自然科学基金资助项目(No:Y2007C130)

摘　　要：目的:探讨喉鳞状细胞癌组织中Ⅰ型胶原(ColⅠ)、Ⅳ型胶原(ColⅣ)、纤维连接蛋白(Fn)的表达及其与肿瘤侵袭和转移的关系。方法:应用免疫组织化学方法检测60例喉鳞状细胞癌患者的癌旁正常组织和癌组织标本中ColⅠ、ColⅣ、Fn的表达,应用VIDAS图像分析仪分析其图像的积分光吸收度(iOD),并进行统计学处理。结果:在癌周正常组织中,ColⅠ表达明显且完整,ColⅣ和基膜Fn表达呈现出一种完整的线状结构,间质Fn则呈完整的网状结构;而在鳞状细胞癌组织中,随分化程度降低,三者的表达逐渐下降,完整性亦逐渐破坏和消失,其变化的差异有统计学意义(P<0.05或P<0.01)。喉鳞状细胞癌组织中ColⅠ、ColⅣ、Fn的表达表现出高度一致性,随着肿瘤体积的增大、病理分级和临床分期的进展、颈淋巴结的转移,其表达均逐渐下降,且表达的变化差异具有统计学意义(P<0.05或P<0.01)。结论:喉鳞状细胞癌组织中ColⅠ、ColⅣ、Fn的表达与肿瘤侵袭和转移等生物学特性密切相关,对了解喉鳞状细胞癌区域性淋巴结转移的可能性、手术方法的选择及预后判断具有一定的意义。Objective：To investigate the expression of collagen typeⅠ（ColⅠ）,collagen type Ⅳ（ColⅣ） and fibronectin（Fn） in laryngeal squamous cell carcinomas（LSCC）and their pathobiological relationship with invasion and metastasis of tumor.Method：The expression of ColⅠ,ColⅣ and Fn was detected by immunohistochemistry method in normal tissue of latero-carcinoma and tissue of carcinoma in 60 specimens of LSCC. Integral optical density（iOD） was detected by image analysis of computer and was analyzed by SPSS13.0.Result：The expression of ColⅠ was obvious and integrity. The expression of ColⅣ and Fn of basal membrane was like intact line-shape appearance and Fn of interstitial substance appeared like a complete network in the normal tissue of latero-carcinoma. Their expression decreased gradually and their integrity was broken and disappeared gradually from well to poorly differentiated LSCC. Their expressions also fell off with the tumor size,clinical stage and cervical lymphatic metastasis gradually and consistently in LSCC. Their variances were statistical significance separately（P〈0.05 or P〈0.01）.Conclusion：The expression of ColⅠ,ColⅣ and Fn was closely related to tumor invasion,the regional lymph node metastasis and other some pathobiological features in LSCC. A detection of ColⅠ,ColⅣ and Fn was of definite significance on a better comprehension of the possibility of metastasis,choice of surgery and prognosis.

关 键 词：喉肿瘤 Ⅰ型胶原 Ⅳ型胶原 纤维连接蛋白 免疫组织化学 

分 类 号：R739.65[医药卫生—肿瘤]