骨髓间充质干细胞移植对大鼠溃疡性结肠炎的修复  

Reparation of bone marrow mesenchymal stem cell-transplantation for the ulcerative colitis rat

作　　者：段征[1] 陈晓云[1] 徐艳华[1] 姜蓉[1] 汪维伟[1] 

机构地区：[1]重庆医科大学组织胚胎学教研室干细胞与组织工程研究室,重庆400016

出　　处：《第四军医大学学报》2009年第23期2761-2764,共4页Journal of the Fourth Military Medical University

基　　金：重庆医科大学自然科学基金落选预研(NSFYY200705)

摘　　要：目的:观察移植骨髓间充质干细胞(BMSCs)对溃疡性结肠炎(UC)大鼠结肠病变的修复.方法:分离培养、荧光标记SD大鼠BMSCs.A,B,C组大鼠制作UC模型,D组为非模型组.4组大鼠均经尾静脉注射1 mL液体:A组为生理盐水;B,D组为BMSCs;C组为加表皮生长因子(EGF)的BMSCs.移植后3,7,14 d各处死5只大鼠,荧光显微镜下观察荧光标记BMSCs在结肠的分布,光镜观察结肠病变以及TNF-α和IL-10的表达.结果:移植第3日各组均可见BMSCs分布于结肠黏膜,病灶处多于正常结肠黏膜.移植第14日,B,C组结肠BMSCs高于D组(P<0.05);与A组比较,B,C组结肠黏膜高表达IL-10,低表达TNF-α,其黏膜损伤修复程度也明显优于A组.B,C组间差异不明显(P>0.05).结论:移植BMSCs可促进UC大鼠结肠损伤的修复.单次给予EGF不能促进BMSCs向病灶的迁移和损伤的修复.AIM：To investigate the reparation of bone marrow mesenchymal stem cell-transplantation for the ulcerative colitis rat.METHODS：The BMSC of SD rat were isolated,cultured and labelled with fluor.SD rats were randomly distributed into 4 groups,Colitis was induced with immune-combined TNBS/ethanol in group A,B and C,but was not induced in group D.Following the induction of colitis,each rat in 4 groups received caudal vein injection of 1 mL fluids,which were：normal sodium for group A,BMSCs for group B and D,BMSCs and EGF for group C.Five rats in each group were sacrificed at day 3,7 and 14 after injection.The distribution of BMSCs in colon was observed with fluorescent microscope;the pathological-changes and expression of TNF-α and IL-10 in colon were observed,too.RESULTS：The fluor marked BMSCs could be seen in the colic mucosa in each group on day 3,and there were more BMSCs in the colonic focus of infection than the normal mucosa.The numbers of BMSCs in the colon of group B and C were more than those in group D（P〈0.05）.Compared with those of group A,there was a higher expression of IL-10 and a lower expression of TNF-α in colonic mucosa of group B and C.The reparation of injuried colonic mucosa in group B and C were obvious surpassed than that in group A,but there was no obviously difference between group B and C.CONCLUSION：Transplantation of BMSCs could promote the reparation of impaired colonic mucosa in UC rat.EGF injected one time could not promote BMSCs migranting to the injured colonic tissue and repairing the damage of colon in UC rat.

关 键 词：骨髓间充质干细胞 移植 溃疡性结肠炎 

分 类 号：R574.1[医药卫生—消化系统] R332[医药卫生—内科学]