高脂饮食大鼠肝脏脂肪性变及NPC1L1基因表达的变化  

Role of Niemann-Pick C1-Like 1 in hepatic steatosis induced by high cholesterol diet

作　　者：蒙广星[1] 袁强[1] 王毅军[1] 孟化[2] 夏琳[3] 白飞虎[4] 

机构地区：[1]天津市第三中心医院肝胆外科,天津300170 [2]首都医科大学附属北京友谊医院胃肠外科,北京100069 [3]第四军医大学消化病研究所,陕西西安710033 [4]宁夏医科大学附属医院消化内科,宁夏银川750000

出　　处：《第四军医大学学报》2009年第23期2794-2797,共4页Journal of the Fourth Military Medical University

摘　　要：目的:探讨NPC1L1及其胆固醇代谢相关基因在高脂饮食诱导肝脏脂肪性变中的可能作用.方法:实验大鼠分为普通饮食组及高脂饮食组,饲喂8周后取肝脏组织,酶法测定肝脏组织中总胆固醇(TC)、磷脂、高密度脂蛋白(HDL)、甘油三酯(TG)含量,RT-PCR检测肝脏组织中NPC1L1,SREBP-1c,FAS及ABCA1基因的表达变化,并在光镜及电镜下观察肝脏病理变化.结果:高脂饮食大鼠肝脏组织TC(67.6±3.1mg/gvs31.8±2.6 mg/g,P<0.01),TG(29.1±2.3 mg/gvs12.5±1.6 mg/g,P<0.01)及磷脂(23.8±2.6 mg/gvs19.8±2.2 mg/g,P<0.05)均较正常饮食大鼠增加.高脂饮食喂养组大鼠肝脏组织中胆固醇代谢相关基因NPC1L1(3.91±0.26,P<0.01),SREBP21c(2.84±0.23,P<0.01)、FASmRNA(6.45±0.25,P<0.01)表达水平显著高于正常饮食组,而ABCA1mRNA(0.56±0.18,P<0.05)表达较正常饮食组降低.结论:肝脏组织NPC1L1表达增加及脂肪代谢相关基因SREBP-1c,FAS。AIM：To determine the role of Niemann-Pick C1-Like 1（NPC1L1） and other genes correlated with cholesterol metabolism in hepatic steatosis induced by high cholesterol diet.METHODS：After being fed with high cholesterol diet for 8 weeks,rats were anaesthetized,livers were weighed and hepatic TC,TG,HDL and phospholipid were extracted and measured by enzymatic methods.Hepatic sterol regulatory element binding protein-1c（SREBP-1c）,fatty acid synthase（FAS）,ATP binding cassette transporter A1（ABCA1） and Niemann-Pick C1-Like 1（NPC1L1） mRNA levels were analyzed by real-time quantitative PCR.Pathological changes were observed by light microscope and electron microscope.RESULTS：Hepatic TC（67.6±3.1 mg/g vs 31.8±2.6 mg/g,P〈0.01）,TG（29.1±2.3 mg/g vs 12.5±1.6 mg/g,P〈0.01） and phospholipid（23.8±2.6 mg/g vs 19.8±2.2 mg/g,P〈0.05）in high cholesterol group markedly increased compared with that in normal cholesterol group.Relative expression of NPC1L1（3.91±0.26,P〈0.01）,SREBP-1c（2.84±0.23,P〈0.01）,FAS mRNA（6.45±0.25,P〈0.01） in high cholesterol group significant enhanced and ABCA1 mRNA（0.56±0.18,P〈0.05） decreased compared with that in normal cholesterol group.CONCLUSION：Enhance of NPC1L1 and disturbance of SREBP-1c,AS,BCA1 might be involved in hepatic steatosis induced by high cholesterol diet.

关 键 词：Niemann-PickC1-Like1 肝脏脂肪性变 实时定量PCR 

分 类 号：R363[医药卫生—病理学]