肝叶钛衣缩紧法快速制备狗肝硬化模型  

Titanium coat for hepatic lobe in the preparation of a hepatic cirrhotic and portal hypertension animal model in dogs

作　　者：曹罡[1] 杨文彬[1] 蔡峰[1] 王永恒[1] 秦兆寅[1] 

机构地区：[1]西安交通大学医学院第二附属医院普外科,陕西西安710004

出　　处：《第四军医大学学报》2009年第23期2876-2878,共3页Journal of the Fourth Military Medical University

摘　　要：目的:应用钛丝网编织技术制作肝叶可缩紧式外衣,简便快速而且较为逼真地模拟肝硬化.方法:选取成年杂种犬20条,术前评价肝功能,测定门脉压力、门脉血流和脾脏大小,活检肝组织.对全部实验犬实行肝两叶切除,其余三叶钛衣缩紧术.术后持续测定肝功能变化4 wk.4 wk末,开腹测定门脉压力及门脉血流变化,观察侧枝循环曲张情况,脾脏增大情况,切取肝组织观察组织学变化.结果:肝叶切除及钛衣缩紧术造成了肝组织数量减少、血流路径明显迂曲、肝叶内炎症反应及胶原增生,较为逼真的模拟了肝硬化的血流和组织学状态.术前肝功能各项指标与术后4 wk末测值比较呈明显的下降趋势,明显低于术前的状态.术后即刻门静脉压力明显高于术前,4 wk末测定门静脉压力亦显著高于术后即刻门静脉压.术后4 wk末,侧枝循环明显曲张,脾脏明显增大.讨论:肝叶钛衣缩紧法可以快速制备狗的肝硬化门脉高压动物模型,耗时仅4 wk.肝二叶切除术完成了肝细胞数量的减少,钛衣缩紧术抑制肝三叶代偿增大,弥漫性钛针钉入促进炎症反应及胶原形成,并且造成了血流路径改变,最终侧枝曲张及脾脏增大,肝功能指标表现出肝硬化特征.AIM：Titanium reticular layers was connected as a coat for hepatic lobes in order to rapidly make a hepatic cirrhosis dog model,which would be much similar to real cirrhosis and portal hypertension for further hepatic research work.METHODS：Twenty healthy cross-breed dogs were selected,and their hepatic function,PVP and PVF,size of the spleen and hepatic tissue were all collected preoperatively as control.The left two lobes of all the dogs were removed,and titanium reticular layers were connected as coats according to the size of the other three lobes.The hepatic function,PVP and PVF,size of the spleen and hepatic tissue were collected again 4 weeks later.RESULTS：Two lobes removal and titanium reticular coats wearing for the other three lobes resulted in reduced hepatic tissue,obviously distorted blood vessels,intrahepatic inflamation and collagen increasing,which distinctly simulate the real blood flow and pathologic changes of hepatic cirrhosis.The hepatic function decreased obviously at the end of the 4th week after the operation,whereas the PVP increased obviously.The varicosities of the branches emerged while the spleen enlarged extraordinarily and the end of the 4th week.CONCLUSION：Titanium coats wearing of hepatic lobes proved to be a good method for the preparation of dog hepatic cirrhosis model.The whole course of the experiment lasted only 4 weeks.The removal of the two lobes resulted in reduced hepatic cells,while the titanium coats limited the compensation of the other three lobes.Titanium needles within the lobes induced the chronic inflammation and increased collagen,which then finally resulted in distorted blood flow within,varicosities,enlarged spleen and decreased hepatic function.

关 键 词：钛 狗 肝硬化 动物模型 

分 类 号：R57[医药卫生—消化系统]