眼附属器淋巴组织增生性病变PCNA和AgNORs的表达及其与组织学分型的关系  

The expression of proliferative cell nuclear antigen and proliferative cell nuclear antigen in ocular aednxal lymphoid hyplasia and its relationship with histopathologic classification

作　　者：魏锐利[1] 蔡季平[1] 叶挺军[1] 朱煌[1] 奚寿增[1] 周浩[1] 

机构地区：[1]第二军医大学附属长征医院眼科,上海200003

出　　处：《肿瘤防治研究》1998年第5期355-357,共3页Cancer Research on Prevention and Treatment

摘　　要：探讨眼附属器淋巴组织增生性病变的增殖活性与组织学分型的关系。材料与方法：应用抗增殖细胞核抗原的单克隆抗体PC10和AgNORs技术对34例眼附属器淋巴组织增生性病变（包括良性淋巴组织增生14例，不典型增生9例，恶性淋巴瘤11例）的石蜡切片进行检测。结果：良性淋巴组织增生与后二者PCNA及AgNORs计数之间均有非常显著的差异（PCNAP＜O．001AgNORsP＜O．01及P＜O．001），不典型增生与恶性淋巴瘤之间PCNA计数相差非常显著（P＜0．01），AgNORS颗粒数量则无显著差异（P＞0．05），同时发现AgNoRS与PCNA计数有良好的相关关系。结论二种技术对眼附属器淋巴组织增生性病变组织学分型有一定意义。Objective to investigate the expression of proliferative cell nuclear antigen and prolifelative cell nuclear antigen in ocular adnexal lymphoid hrplasia and its relationship with histopathologic classification. Method Using anti-proliferativfe cell nuclear antigen monoclonal antibody (pc10) and AgNORs method, the proliferative cell in 34 cases of ocular adnexal lymphoid neoplasms (benign lymphoid lesion, BLL, 14 cases; atypical lymphoid hyperplasias, AH, 9 cases; malignant lymphomas,ML, 11 cases) were detected in formalin fixed and paraffinembeded tissuse. Results A significant difference between BLL and AH BLL and ML was observed (PCNAP < 0.01, AgNORs P < 0.01, P< 0.001 ); similar result was observed between AH and ML in PCNA index (P < 0.01). But there was not a sugnificant difference in AgNORs count (P > 0. 05). The linear regression coefficient betWeen PCNA indeX and AgNORs number was excellent(0.8693). Conclusion The twO methords may be helpful to distinguish ocular adnexal lymphoid reactive hyplasia and malignant lymphoma. Our study is the first regarding the comparative assessment in ocular adnexal lymphoid hyplasia.

关 键 词：眼肿瘤 眼附属器 淋巴组织增生 PCNA AGNOR染色 

分 类 号：R777.04[医药卫生—眼科] R739.709[医药卫生—临床医学]