华东地区散发性戊型肝炎病毒系统进化分析  

Phylogenetic analysis of sporadic hepatitis E virus in Eastern China

作　　者：夏玉刚[1] 李燕婷[2] 陆一涵[1] 任红[2] 胡安群[3] 朱建福[4] 王学才[4] 姜庆五[1] 郑英杰[1] 

机构地区：[1]复旦大学公共卫生学院流行病学教研室教育部公共卫生安全重点实验室,上海200032 [2]上海市疾病预防控制中心 [3]安徽省安庆市立医院 [4]浙江省德清县疾病预防控制中心

出　　处：《中华流行病学杂志》2009年第12期1269-1272,共4页Chinese Journal of Epidemiology

基　　金：基金项目：国家自然科学基金（30771842,30872158）;上海市卫生局科研课题（2006100）;上海市重点学科项目（B118）

摘　　要：目的 了解华东地区散发性戊型肝炎病毒（HEV）的系统进化特征。方法2005—2008年收集华东地区14家二级或三级医院413份散发戊型肝炎患者血清，应用巢式RT-PCR方法检测HEVRNA并测序。参照GenBank相关序列，分析各序列之间的同源性，并构建进化树。结果413例患者的男女性别比为1．75：1，40～69岁患者居多（61．5％）。413份患者血清有140份分离出HEVRNA，阳性分离率为34％。所有分离的140份病毒株均属于HEVⅣ型，与Ⅰ、Ⅱ、Ⅲ和Ⅳ型参考病毒株的核苷酸同源性分别为77．9％～88．3％、80．8％～90．6％、73．4％～85．2％和91．0％～95．4％。结论基因Ⅳ型HEV是引起华东地区散发性戊型肝炎的优势病毒株，其起源和进化等问题仍需进一步研究。Objective To-understand-phylogenetic characteristics of sporadic hepatitis E virus （HEV） in eastern China. Methods Four hundred and thirteen sera were collected from sporadic hepatitis E cases in 14 secondor first-class hospitals in Eastern China from 2005 to 2008 and detected with a nested RT-PCR assay. Partial nucleotide sequences of the HEV isolates were determined for phylogenetic analysis with propotype sequences in the GenBank. Results The male-to-female sex ratio of the patients was 1.75：1 with 61.5% of them aged 40-69 years old. HEV RNA was detected in 140 out of 413 （34%） serum samples. Phylogenetic analysis revealed that all the 140 HEV isolates belonged to genotype IV, sharing 77.9%-88.3%, 80.8%-90.6%, 73.4%-85.2% and 91.0%-95.4% nucleotide sequence identities with prototype Ⅰ , Ⅱ , Ⅲ and Ⅳ HEV isolates respectively. Conclusion It was evident that genotype IV HEV served as the main causative agent of sporadic HEV infection in Eastern China. However the viral origin and evolution needs further clarification.

关 键 词：戊型肝炎病毒 系统进化 基因型 

分 类 号：R51[医药卫生—内科学]