后根切断术及辣椒素对大鼠脊髓后角δ阿片受体免疫反应性的影响  

The Effects of Dorsal Rhizotomy and Capsaicin on ?Opioid Receptor Immunoreactivity in the Spinal Dorsal Horn of the Rat

作　　者：王小丽[1] 张亦农[1] 李和[1] 

机构地区：[1]同济医科大学基础医学院组织学与胚胎学教研室,武汉430030

出　　处：《同济医科大学学报》1998年第5期364-366,共3页Acta Universitatis Medicinae Tongji

基　　金：国家自然科学基金资助项目(No.39770386)

摘　　要：应用免疫组织化学方法观察大鼠单侧脊髓后根切断后和蛛网膜下腔注射辣椒素后,脊髓后角浅层δ阿片受体(DOR)免疫反应性的变化。结果显示:①在单侧后根切断术后第5d,所有大鼠术侧脊髓后角I～Ⅲ层内DOR的免疫反应性均较对照侧明显减弱(P<0.01);②在所有辣椒素处理大鼠中,其脊髓后角浅层DOR免疫反应性均较对照组明显下降(P<0.01)。结果提示,脊髓后角浅层内部分DOR来源于一级传入神经元,其中部分为传入伤害性信息的神经元。The changes of the immunoreactivity of 8 opioid receptor (DOR) in the superficial layers of the spinal dorsal horn of the rat were observed by using immunohistochemistry after unilateral dorsal rhizotomy or subarachnoid injection of capsaicin. The DOR-immunoreactivity in the laminae I^III of the spinal cord on the side ipsilateral to the dorsal rhizotomy was markedly weakened on the day 5 after operation (P< 0, 01). Moreover,the DOR- immunoreactivity in the same layers of the rat was highly reduced on the day 7 after subarachnoid administration of capsaicin,compared with that treated with vehicle(P<0. 01). These re-sults indicate that part of DORs in the superficial layers originates from primary afferent neurones,some of which are the neurones contributing to the input of nociceptive information.

关 键 词：阿片受体 后根切断术 辣椒素 C纤维 脊髓后角 

分 类 号：R338.3[医药卫生—人体生理学] R966[医药卫生—基础医学]