反相高效液相色谱法测定蜂胶水提物中的酚类化合物  

Analyses of Phenolic Compounds of Water Extract from Propolis by Reversed Phase High Performance Liquid Chromatography

作　　者：陈滨[1] 罗丽萍[1] 丽艳[1] 徐元君[1] 付宇新[1] 高荫榆[1] 

机构地区：[1]南昌大学生命科学与食品工程学院,南昌330031

出　　处：《分析化学》2009年第12期1786-1790,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.C30700105);江西省青年科学家(井冈之星)培养计划(No.S00590)资助

摘　　要：建立了同时测定中国蜂胶水提物中23种酚类化合物的反相高效液相色谱(RP-HPLC)分析方法。采用ZORBAX Eclipse XDB C18色谱柱(150 mm×4.6 mm×5μm);流动相:甲醇-0.1%甲酸,梯度洗脱,流速1.0 mL/min,检测波长为256和280 nm,进样量20μL,柱温35℃。各对照品质量浓度与色谱峰面积线性关系良好,具有较好的精确度和重现性,标准加入回收率在93.29%～106.61%之间。采用此法,从河北蜂胶中检测出18种对照品,其中表儿茶素含量最高35.50 mg/g WEP,其次是3,4-二甲氨肉桂酸15.41 mg/g WEP。而云南蜂胶中检测到9种对照品,表儿茶素含量最高11.23 mg/g WEP,其次是白杨素3.61 mg/g WEP,两地蜂胶WEP的HPLC图相似度分别为0.099(256 nm)和0.194(280 nm),化学成分差异明显。A reversed phase high-performance liquid chromatography method for the simultaneous analysis of 23 kinds of phenolic components in water extract of porpolis （WEP） was developed. The separation was performed on ZORBAX Eclipse XDB C18 column （150mm×4.6mm×5μm） by gradient elution. The mobile phase consisted of ethanol with 0.1% formic acid at the flow rate of 1.0 mL/min. The detection wavelengths were 256 and 280 nm. The injection volume was 20μL, and the column temperature was maintained at 35℃. The method showed good linear relationship, precision and repeatability. The recoveries were between 93.3% and 106.6%. Eighteen reference compounds were detected in WEP of Hehei by this method, The content of Cateehin was the highest （30.50 mg/g）, the next was 3,4-dimethoxycinnamie acid （15.41 mg/g）. Nine reference compounds were detected in WEP of Yunnan. The content of eatechin was also the highest （11.23 mg/g）, the next was chrysin （15.41mg/g）. Similarity of WEPs between the products of Hebei and Yunnan were 0.099 （256 nm） and 0.194 （280 nm）. The chemical compositions of two WEPs were remarkably different.

关 键 词：反相高效液相色谱法 蜂胶 水提物 酚类化合物 

分 类 号：R284[医药卫生—中药学]