纳米颗粒钛膜对成骨细胞合成功能的影响  

Effect of nano-granule titanium films on synthesis of osteoblasts

作　　者：巴凯[1] 张静[1] 王虎[1] 刘媛媛[1] 杨振宇[1] 李明霞[1] 李伟[1] 缪竞威[2] 

机构地区：[1]口腔疾病研究国家重点实验室,四川大学,四川成都610041 [2]四川大学原子核科学技术研究所,四川成都610064

出　　处：《华西口腔医学杂志》2009年第6期592-594,共3页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(10675087和10574095)

摘　　要：目的通过对比不同纳米颗粒钛膜表面成骨细胞的合成能力,评价其生物相容性。方法采用直流磁控溅射法通过控制温度(常温、100℃、250℃、380℃)构建4级纳米颗粒钛膜,将SD乳鼠第3代成骨细胞接种于其表面及未镀膜的钛片表面,对表面成骨细胞上清液中骨钙素(OC)含量进行检测。结果在7、14d时,随着时间的延长,各实验组和对照组样本表面的细胞上清液中OC含量均增大。在7d时,对照组与其他各组相比,OC含量间差异均有统计学意义(P<0.05)。在14d时,100℃实验组表面OC含量增幅最大,100℃实验组与380℃实验组、对照组、空白组相比,其差异具有统计学意义(P<0.05),250℃实验组与380℃实验组、对照组、空白组相比,其差异也具有统计学意义(P<0.05)。结论钛表面纳米改性有利于其生物相容性,不同纳米粒径可以影响成骨细胞的合成功能。Objective To compare the synthetic ability of osteoblasts on the surface of different nano-granule titanium films and investigate the correlation between nanophase titanium films and cellular biocompatibility. Methods Four different nano-granule titanium films were produced by direct current magnetron sputtering, at ambient, 100 ℃, 250 ℃, 380 ℃ substrate temperature, respectively. Rat osteoblasts were seeded on the surface of four treated groups of titanium film samples and non-treated Ti sample （control group）. The production of osteocalcin （OC） in all five groups were detected by using double antibody sandwich enzyme-linked immunosorbent assay. Results The production of OC increased gradually from day 7 to day 14 in all groups. In the control group, it showed significant differences with other five groups on day 7. On day 14, the production of OC in 100 ℃ group was the highest, and it showed significant differences with 380 ℃, control group and blank group. In 250 ℃ group, the production of OC also showed significant differences with 380 ℃, control group and blank group（P〈0.05）. Conclusion Titanium with nano-modified surface had good biocompatibility and different nano-granule titanium films could affect the synthesis of osteoblasts.

关 键 词：纳米结构 钛膜 成骨细胞 骨钙素 生物相容性 

分 类 号：R783.1[医药卫生—口腔医学]