一氧化氮合酶在犬下颌骨牵张成骨过程中的表达和意义  

Expression and significance of nitric oxide synthase in mandibular distraction osteogenesis of canine

作　　者：周诺[1] 廖妮[1] 韦山良[1] 梁飞新[1] 

机构地区：[1]广西医科大学口腔医院口腔颌面外科,广西南宁530021

出　　处：《华西口腔医学杂志》2009年第6期676-680,共5页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(30760273);广西科学基金资助项目(桂科基0448058);广西"新世纪十百千人才工程"专项资金资助项目(2004223)

摘　　要：目的研究一氧化氮合酶(NOS)在犬下颌骨牵张成骨及骨创伤修复过程的表达和意义。方法28只犬随机分成牵张组和直接延长组各12只及正常对照组4只,用免疫组化法检测牵张第6天、牵张后固定2周和8周NOS表达水平的变化。结果牵张第6天牵张组可见牵开区组织内炎性细胞浸润,血管周围和间质内较多红细胞漏出。牵张及固定早期,牵张组和直接延长组诱导型NOS(iNOS)与内皮型NOS(eNOS)阳性表达均明显高于正常对照组,直接延长组的iNOS和eNOS阳性表达低于牵张组,差异均有统计学意义(P<0.05);牵张后固定8周iNOS和eNOS阳性表达3组间比较差异无统计学意义(P>0.05)。结论NOS在牵张早期表达升高,结合在牵张早期组织内红细胞漏出,提示牵张成骨过程中存在某种程度的微创伤,这种微创伤可能是牵张成骨的重要启动因素之一。Objective To investigate the expression and significance of nitric oxide synthase（NOS） during mandibular distraction osteogenesis and bone trauma healing, to futher study the mechanism of distraction osteogenesis. Methods Twenty-eight adult dogs were randomly divided into DO group （12 dogs）, acutely lengthening group （12 dogs） and control group（4 dogs）. Immunohistochemical examination were carried out to test the expression of NOS during the sixth day in distraction period, the second and eighth week of consolidatiorL Results In DO group infiltration of inflammatory cell was found in the distraction gap, more red blood cells CRBC） leak out around vascellum and matrix in the sixth day in distraction period. The expression of local iNOS （inducible NOS） and eNOS （endothelinal NOS） in DO gruoup and acutely lengthening group was higher than that in the control group（P〈0.05）, the expression of local iNOS and eNOS in acutely lengthening group was lower than that in DO group（P〈0.05） in the early of distraction period and the consolidation. The expression of local iNOS and eNOS was no statistic difference between three groups （P〉0.05） in the eighth week of consolidation. Conclusion NOS as a sensitive index of tissue trauma are highly expressed, and RBC was found leaking out in the early of distraction, indicating that micro-trauma to some extent may occurr during DO procedure, the micro-trauma may be one of the significant factors which increasing regeneration of bone during DO.

关 键 词：牵张成骨 一氧化氮合酶 创伤 

分 类 号：R782.2[医药卫生—口腔医学]