microRNA-206对人横纹肌肉瘤细胞增殖的影响  

Effect of microRNA-206 on proliferation of human rhabdomyosarcoma cells

作　　者：陈晓燕[1] 王教[1] 王瓯晨[2] 

机构地区：[1]温州医学院眼视光学院,浙江温州325027 [2]温州医学院附属第一医院,浙江温州325000

出　　处：《实用肿瘤杂志》2009年第6期538-541,共4页Journal of Practical Oncology

摘　　要：目的研究microRNA-206在人横纹肌肉瘤细胞A673和A204中的表达以及对细胞增殖的影响。方法Northern blotting检测正常肌肉组织与人横纹肌肉瘤细胞中microRNA-206表达水平的差异,MTS法检测转染microRNA-206后对细胞增殖的影响。Western blotting检测microRNA-206后对细胞中蛋白水平的影响。结果Northern blotting结果显示,microRNA-206在正常肌肉组织中高表达,在横纹肌肉瘤细胞A673和A204中不表达;MTS结果表明转染microRNA-206后,横纹肌肉瘤细胞的数目明显减少,microRNA能够明显下调人横纹肌肉瘤细胞中p-ERK的表达。结论microRNA-206通过ERK信号传导通路抑制横纹肌肉瘤细胞的增殖。Objective To investigate the effect of microRNA-206 on the proliferation of human rhabdomyosarcoma cell line A673 and A20.1. Methods MicroRNA-206 was transfected into human rhahdomyosarcoma A673 and A204 cells. Northern blotting was performed to detect the expression level of microRNA-206. The proliferation of rhabdomyosarcoma cells was examined by MTS. Western blotting was performed to detect the expression level of protein. Results Northern hlotting showed that microRNA-206 was highly expressed in human skeletal muscle but not in rhabdomyosarcoma A673 and A204 cells. After transfection of microRNA-206 ,the proliferation of A673 and A206 ceils was inhibited and the expression of p-ERK was downregulated. Conclusion microRNA-206 can suppress the proliferation of rhabdomyosarcoma cells by downregulation of p-ERK.

关 键 词：横纹肌肉瘤 微RNAS 基因疗法 增生 转染 基因表达 

分 类 号：R738.7[医药卫生—肿瘤]