CCK-8上调LPS诱导的大鼠肺组织HO-1表达的信号转导机制  

CCK-8 up-regulats signal pathway of LPS-induced HO-1 expression in rat lungs

作　　者：黄新莉[1] 周晓红[1] 周君琳[2] 羡晓辉[1] 丁春华[1] 

机构地区：[1]河北医科大学病理生理教研室,河北石家庄050017 [2]首都医科大学附属北京朝阳医院骨科,北京100020

出　　处：《中国病理生理杂志》2009年第12期2390-2393,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30271337)

摘　　要：目的:旨在研究八肽胆囊收缩素(CCK-8)上调脂多糖(LPS)诱导的大鼠肺组织中血红素氧合酶(HO)-1表达的信号转导机制。方法:将42只雄性SD大鼠随机分为7组(每组6只),即对照组、LPS组、LPS+SP600125(JNK特异性抑制剂)组、CCK-8+LPS组、CCK-8+LPS+SP600125组、CCK-8组、CCK-8+SP600125组。注药后6h放血处死动物留取肺组织,应用RT-PCR、Western blotting和免疫荧光流式细胞术(FCM)等技术分别检测各组肺组织HO-1mRNA和蛋白表达。结果:与正常对照组相比,LPS组可见肺组织中出现明显的HO-1 mRNA表达的阳性信号,CCK-8可使LPS诱导的阳性表达信号进一步增强,CCK单独作用也可上调HO-1表达。信号密度扫描结果显示,LPS组、CCK-8+LPS组和CCK-8组HO-1 mRNA表达强度分别是正常对照组3.01(P<0.01)、5.88(P<0.01)和3.45倍(P<0.01);JNK特异性抑制剂SP600125抑制了CCK-8和(或)LPS诱导的HO-1mRNA表达;Western blotting、免疫荧光FCM检测结果显示,肺组织HO-1蛋白表达变化与其mRNA表达一致。结论:JNK/c-Jun通路在CCK-8上调LPS诱导肺组织HO-1表达过程中发挥重要作用。AIM ： To study the signal pathway involved in up - regulation of LPS - induced HO - 1 expression by CCK - 8. METHODS ： Forty - two SD rats were divided into 7 groups （ six rats each） randomly as follows ： control group, LPS group, LPS ＋ SP600125 （JNK - specific inhibitor） group, CCK - 8 ＋ LPS group, CCK - 8 ＋ LPS ＋ SP600125 group, CCK - 8 group and CCK - 8 ＋ SP600125 group. Lungs from the rats in these 7 groups were excised 6 h after the a- gents were administered. HO - 1 mRNA expression was examined by RT - PCR. The protein expression of HO -1 was detected by Western blotting and immunofluorescence flow cytometry （FCM）. RESULTS ： There were significant positive expression of HO - 1 mRNA in LPS group compared to control group. CCK - 8 enhanced LPS - induced HO - 1 mRNA expression and CCK - 8 alone induced HO - 1 mRNA expression as well. The mRNA expressions of HO - 1 in LPS group, CCK - 8 ＋ LPS group and CCK - 8 group were 3.01 （ P 〈 0. 01 ）, 5.88 （ P 〈 0. 01 ） and 3.45 （ P 〈 0.01 ） times as many as that in control group, respectively. SP600125 inhibited the mRNA expression of HO -1 induced by CCK- 8 and （or） LPS. The change of HO - 1 protein expression was in accordance with that of HO - 1 mRNA expression by Western blotting and immunofluorescence FCM. CONCLUSION ： These results suggest that JNK/c - Jun signal pathway plays an important role in the up - regulation of LPS - induced HO - 1 expression by CCK - 8.

关 键 词：脂多糖类 胆囊收缩素 血红素氧合酶-1 信号转导 

分 类 号：R363.2[医药卫生—病理学]