CYP1A1基因多态性与宫颈鳞癌的关系  

The relationship between polymorphisms of CYP1A1 gene and cervical squamous carcinoma

作　　者：李爱华[1] 孔爱荣[1] 张圣红[1] 

机构地区：[1]聊城市人民医院妇产科,聊城252000

出　　处：《现代妇产科进展》2009年第11期828-831,共4页Progress in Obstetrics and Gynecology

摘　　要：目的:检测宫颈鳞癌组织中CYP1A1等位基因的表达,探讨CYP1A1基因多态性在宫颈鳞癌发生中的作用。方法:收集宫颈鳞癌组织标本50例,以正常宫颈组织61例为阴性对照。采用等位基因特异性PCR法(ASA-PCR)和PCR扩增限制酶切法(RFLP-PCR)检测宫颈鳞癌组织中CYP1A1等位基因Exon7位点和MspⅠ位点多态性;用SPSS13.0软件建立数据库,进行χ2检验、logistic回归分析。结果:(1)CYP1A1Exon73种多态基因型在宫颈鳞癌组和对照组分布差异有统计学意义(P<0.005),宫颈鳞癌组Ile/Val、Val/Val基因型的分布频率明显高于对照组。Ile/Val和Val/Val基因型的个体发生宫颈鳞癌的OR值分别是Ile/Ile基因型个体的1.969倍和3.15倍,差异有统计学意义(P<0.05);(2)CYP1A1MspⅠ位点多态性分析:将MspⅠ位点的PCR产物行酶切分析,显示m1/m2杂合型、m2/m2突变型在宫颈鳞癌组和对照组表达差异无统计学意义(P>0.05)。结论:宫颈鳞癌组织中CYP1A1基因Exon7位点突变率升高,Ile/Val和Val/Val基因突变型个体发生宫颈鳞癌的危险性明显升高;CYP1A1基因MspⅠ位点的多态性可能与宫颈鳞癌易感性无关。Objective：To examine the polymorphisms expression of the CYP1 A1 gene in cervical squamous cancer and explore the relationship between CYP1A1 polymorphisms and cervical squamous cancer. Methods：Fifty cervical squamous cancer cases were collected and 61 normal cervix tissue samples were taken as control. All cases had not accepted chemotherapy or radiotherapy. The 7th exon and Msp I polymorphisms of CYP1A1 were detected in these tissue by PCR-RFLP and ASA-PCR. Statistical analysis was performed using SPSS13.0 statistics software. Results： （ 1 ） The frequencies of the 7th exon of CYP1 A1 polymorphisms in cancer group were significantly different from that of the control group, the frequencies of Ile/Val and Val/ Val genotype in cancer group were significantly higher than that in the control group. The odds ratio of cervical squamous cancer with Ile/Val and Val/Val genotypes were 1. 969 and 3.15 than that of the Ile/Ile genotype（P〈 0.05 ）. （2）There was no significant correlation between Msp I polymorphism and cervical squamous cancer susceptibility. Conclution：The individuals with Ile/Val or Val/Val genotype of the 7th exon of CYP1 A1 have higher susceptibility of cervical squamous cancer than that of the Ile/Ile genotype. The polymorphism of Msp I is not associated with susceptibility of cervical squamous cancer.

关 键 词：宫颈肿瘤 细胞色素P450 CYP1A1 等位基因特异性PCR法 PCR扩增限 制酶切法 

分 类 号：R737.33[医药卫生—肿瘤]