GSTM1、CYP2E1基因多态性与新疆维吾尔族妇女宫颈癌及癌前病变关联性的研究  

Association between genetic polymorphism of GSTM1,CYP2E1 and susceptibility to cervical cancer and its precancerous lesions in Uighur women in Xinjiang

作　　者：刘瑶[1] 马文静[2] 刘清[2] 杨婷[2] 徐淑永[2] 马彩玲[1] 

机构地区：[1]新疆医科大学第一附属医院妇产科,乌鲁木齐830054 [2]新疆医科大学第一附属医院医学研究中心,乌鲁木齐830054

出　　处：《现代妇产科进展》2009年第11期840-843,共4页Progress in Obstetrics and Gynecology

摘　　要：目的:探讨谷胱甘肽硫转移酶基因GSTM1和细胞色素氧化酶P4502E1(CYP2E1)的基因多态性与新疆维吾尔族妇女宫颈癌前病变与癌的关联性。方法:用聚合酶链反应(PCR)方法及聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法为21例浸润性宫颈癌(ICC)、41例宫颈上皮内瘤变(CIN)及45例慢性宫颈炎标本进行GSTM1和CYP2E1RsaⅠ位点基因分型。结果:ICC组和CIN组的GSTM1空白基因型频率明显高于慢性宫颈炎组(对照组),差异有统计学意义(χ2=7.56,P<0.05;χ2=13.30,P<0.05),携带GSTM1(-)的个体患宫颈癌的危险性比携带GSTM1(+)的个体升高3.47倍(OR=4.47,95%CI=1.5～13.0),患CIN的危险性升高4.30倍(OR=5.30,95%CI=2.2～13.0)。CYP2E1基因RsaⅠ位点多态在3组的频率差异无统计学意义(χ2=2.28,P>0.05)。联合分析3组病例CYP2E1基因RsaⅠ多态性和GSTM1基因多态性,未发现两者之间的交互作用与ICC及CIN易感性有关(χ2=4.47,P>0.05)。结论:GSTM1基因空白型可能与维吾尔族宫颈癌及癌前病变风险增加有关,CYP2E1基因RsaⅠ多态性与宫颈癌及癌前病变无关。Objective： To study the association between genetic polymorphism of glutathione S-transferase classes mu （ GSTM1 ） and/or cytochrome P450 2El （ CYP2EI ） and susceptibility to cervical cancer and its precancerous lesions in Xinjiang Uighur women. Methods：Genetic polymorphisms of GSTM1 and CYP2E1 were detected in 21 cases of invasive carcinoma of cervix（ICC） ,41 cases of cervical intraepithelial neoplasia（CIN）and 45 cases of chronic cervi- citis without ICC and CIN （as controls）among Uighur women in Chinese Xijiang using polymerase chain reaction （PCR） method and polymerase chain reactionrestriction fragment length pol- ymorphism （PCR-RFLP） technique. Results： The frequency of GSTM1-null genotype in ICC and CIN group was significantly higher than that in chronic cervicitis （ χ2= 7.56, P 〈 0.05 ; χ2 = 13.30 ,P〈0.05 ,respectively）. The individuals carrying GSTMI-null genotype showed a 3.47- fold increase of ICC risk （ OR 4.47,95 % CI 1.5 - 13.0） and 4.3-fold increase of C1N risk （ OR =5.30,95% CI 2.2 -13.0）respectively,compared with the individuals carrying GSTMl-posi- tive genotype. No significant difference in three Rsa I genotype distribution of CYP2E1 was found in three groups（ χ2= 2.28, P〉0.05）. When the combination of polymorphism of CYP2E1 Rsa I genotype and GSTMI genotype was analyzed, there was no significant difference in the distribution of combination of CYP2EI C1/C1 and GSTM1 null genotypes in the three groups. Conelusion：GSTM1 null genotype maybe associaied with increased risk of cervical cancer and its precancerous lesions in Uighur women. Risk of cervical cancer and its precancerous lesions is not associated with the CYP2E1 genetic polymorphism.

关 键 词：宫颈肿瘤 宫颈上皮内瘤样病变 GSTM1 CYP2E1 基因多态性 维吾尔族 

分 类 号：R737.33[医药卫生—肿瘤]