一氧化氮合成酶抑制剂对缺血纹体多巴胺释放的影响  

The influence of NOS inhibitor on the releases of dopamine from ischemic striatums

作　　者：张绍东[1,2] 程兰英[1,2] 徐超 徐亚萍 崔雯[1,2] 

机构地区：[1]北京市神经外科研究所 [2]中日友好医院临床医学研究所

出　　处：《中华神经外科杂志》1998年第5期303-305,共3页Chinese Journal of Neurosurgery

基　　金：国家"八五"攻关课题

摘　　要：目的：研究一氧化氮合成酶抑制剂（Ｌ－ＮＮＡ）对大鼠半球持续缺血时纹体多巴胺（ＤＡ）释放的影响。方法：阻断大鼠基底动脉和双侧颈总动脉作为半球缺血模型，用微透析法结合高压液相法测定半球缺血３０，６０，９０，１２０分钟时大鼠纹体ＤＡ的释放。结果：大鼠半球缺血３０分钟时，纹体ＤＡ的释放形成高峰，其后逐渐下降，但都高于缺血前释放量。Ｌ－ＮＮＡ能明显抑制缺血时纹体ＤＡ的不正常释放。结论：ＮＯ参与了缺血时纹体ＤＡ释放的调节。Ｌ－ＮＮＡ抑制半球缺血时ＤＡ的不正常释放可能是其保护缺血大脑的机制之一。Objective: To study the influence of NOS inhibitor L NNA on the release of dopamine from rat striatums during persistent global ischemia. Methods:The bilateral carotid arteries and basilar arteries of rats were occluded as the model of global ischemia. The releases of DA were determined by microdialysis and HPLC methods. Results:The peak of DA release from rat striatum was formed during 30 min global ischemia,then,it was decreased gradually. However,all of the DA releases during 60,90,120min global ischemia were significantly higher than that before ischemia. The abnormal release of DA from ischemic striatums were significantly inhibited by L NNA. Conclusion:NO is involved in the regulation of DA release from striatum during cerebral ischemia. The abnormal DA release inhibited by L NNA during global ischemia may be one of the protective mechanism of this agent on brain ischemia.

关 键 词：半球缺血 纹体 多巴胺 L-NNA 脑缺血 

分 类 号：R743.31[医药卫生—神经病学与精神病学]