HPLC法同时测定川芎药材中3种有效成分的含量  

Determination of ferulic acid,6,7-di-hydroxyligustilide and 4-hydroxy-3-butylphthalide in Rhizoma Chuanxiong by HPLC

作　　者：王漪檬[1] 张晖芬[1] 闫宝庆[1] 陈晓辉[1] 毕开顺[1] 

机构地区：[1]沈阳药科大学药学院药分教研室,沈阳110016

出　　处：《药物分析杂志》2009年第12期2109-2112,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立HPLC法同时测定川芎药材中阿魏酸、6,7-二羟基藁本内酯和4-羟基-3-丁基苯酞三组分含量的方法。方法:采用SHINWA-ODS(250mm×4.6mm,5μm)色谱柱,流动相为甲醇(A)-0.1%醋酸溶液(B),梯度洗脱(0～20min,A:30→60;20～33min,A:60→70);流速:1.0mL·min-1;检测波长:DAD多波长检测,阿魏酸为278nm、6,7-二羟基藁本内酯320nm、4-羟基-3-丁基苯酞254nm。结果:三组分的检测范围分别为阿魏酸:7.15～71.50ng·mL-1(r=0.9999)、6,7-二羟基藁本内酯:39.90～399.00ng·mL-1(r=0.9997)、4-羟基-3-丁基苯酞:0.29～2.88ng·mL-1(r=0.9998);平均回收率(n=9)分别为阿魏酸103.2%(RSD=1.9%);6,7-二羟基藁本内酯102.3%(RSD=1.7%);4-羟基-3-丁基苯酞102.2%(RSD=2.0%)。结论:该法操作简单,结果准确,重现性好,为各多组分评价不同产地川芎药材的质量提供了可靠的分析方法。Objective：To develop an HPLC method for ferulic acid,6,7-di-hydroxyligustilide and 4-hydroxy-3-butylphthalide in Rhizoma Chuanxiong.Methods：A SHINWA-ODS（250 mm × 4.6 mm i.d,5 μm）column was used to separate the sample at the column temperature of 30 ℃.Methanol was mobile phase A and 0.1% aetic acid was mobile phase B with gradient elution （0-20 min,A：30→60;20-33 min,A：60→70） at a flow rate of 1.0 mL·min-1.The detector was DAD.The detection wavelengths of ferulic acid,6,7-di-hydroxyligustilide,4-hydroxy-3-butylphthalide were at 320 nm,278 nm and 254 nm.Results：The calibration curve was linear over the concentration of 7.15-71.50 ng·mL-1 for ferulic acid （r=0.9999）;39.90-399.00 ng·mL-1 for 6,7-di-hydroxyligustilide（r=0.9997） 0.29-2.88 ng·mL-1 for 4-hydroxy-3-butylphthalide（r=0.9998）.The average recovery of ferulic acid;6,7-di-hydroxyligustilide and 4-hydroxy-3-butylphthalide were 103.2%,102.3%,102.2% and the RSD were 1.9%,1.7%,2.0%,respectively.Conclusion：This method is smiple and reproducible,which can be used for the quality control of Rhizoma Chuanxiong.

关 键 词：HPLC 阿魏酸 6 7-二羟基藁本内酯 4-羟基-3-丁基苯酞 川芎 

分 类 号：R917[医药卫生—药物分析学]