大豆皂甙抗血浆脂质过氧化和红细胞保护作用的研究  

Anti-plasma Lipid Peroxidative and Erythrocyte Protective Effects of Soya-saponins

作　　者：李天[1] 刘淑萍[1] 全吉淑[1] 

机构地区：[1]延边大学医学部,吉林延吉133000

出　　处：《大豆科学》2009年第6期1067-1070,共4页Soybean Science

基　　金：吉林省科技发展计划资助项目(No.200705428)

摘　　要：用分光光度法测定大豆皂甙的总抗氧化能力(T-AOC)和抗活性氧能力(ROSSC),用八木变法测定血浆过氧化脂质(LOOH)含量,用硫代巴比妥酸(TBA)法测定血浆丙二醛(MDA)含量,用分光光度法测定红细胞溶血程度,以期研究大豆皂甙的体外抗脂质过氧化和红细胞保护作用。结果表明:大豆皂甙具有较强的体外总抗氧化能力和抗活性氧能力,可抑制血浆和红细胞膜脂质过氧化作用,减少红细胞溶血程度。说明大豆皂甙具有体外抗血浆脂质过氧化和红细胞保护作用。The anti-lipid peroxidative and erythrocyte protective effects of soyasaponins were investigated in vitro. The total antioxidant activity（T-AOC）and the reactive oxygen species scavenging capacity（ROSSC） were detected by spectrophotometric method,the content of lipid hydroperoxide（LOOH） was measured by Yagi＇s method,the content of malonyldialdehide （MDA） was measured by thiobarbituric acid （TBA）assay, the hemolysis of erythroeytes was detected by spectrophotometric method. The result showed that soyasaponins exerted the high total antioxidant activity and the ROS scavenging acitivity in vitro, inhibited the lipid peroxidation of plasma and erythrocyte membrane, and suppressed the hemolysis of erythrocytes. It is suggested that soyasaponins have the anti-plasma lipid peroxidative and erythrocyte protective effects in vitro.

关 键 词：大豆 皂甙 体外 脂质过氧化 红细胞保护 

分 类 号：R285.5[医药卫生—中药学]