猪肉组织色素浓度的近红外无损检测  

Noninvasive Detection of the Concentrations of Pigments in Pork Tissue Using Near Infrared Spectroscopy

作　　者：腾轶超[1] 李岳[1] 黄岚[2] 丁海曙[1] 

机构地区：[1]清华大学医学院生物医学工程系,北京100084 [2]中国农业大学信息与电气工程学院,北京100083

出　　处：《光谱学与光谱分析》2010年第1期66-69,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(60578004);国家高技术研究发展计划项目(2007AA10Z212);中国博士后科学基金项目(20070410514)资助

摘　　要：基于血红蛋白、肌红蛋白等色素在近红外波段的吸收谱,采用近红外光谱(NIRS)技术,通过检测出射光相对入射光的衰减,可解算生物组织中上述色素的浓度。基于稳态空间分辨的NIRS研发了可检测组织中色素浓度的装置,该装置的传感器包括一个可发射3个不同波长的LED光源,以及与LED位于同一直线上且距离分别为30和40 mm的2个检测器。用该装置检测了2块猪肉组织(分别为新鲜的猪背长肌和股直肌)的色素浓度,结果表明,背长肌和股直肌中血红蛋白、肌红蛋白等色素的总浓度分别为(6.42±1.51)μmol·L^(-1)和(15.48±4.54)μmol·L^(-1),接近于现有报道的经验值。在无损、实时、直接、简便检测猪肉组织的色素浓度方面,上述NIRS方法及装置是可信的。Based on the absorption spectra of hemoglobin and myoglobin in the near infrared band, the concentrations of these pigments in the biological tissues can be obtained using near infrared spectroscopy （NIRS） by detecting the intensity attenuation of the emitted light compared with the incident light. Based on the steady-state spatially resolved NIRS, the prototype for detecting the concentrations of tissue hemoglobin and myoglobin was independently developed by our group. The probe consisted of an LED light source which could emit three different wavelengths in the near infrared band, and two detectors which were placed on the same line with and at the distances of 30/40 mm to the LED. The pigment concentrations of two pieces of pork, one from the erector spinae and the other from the rectus femoris, were detected using this prototype. The total concentrations of hemoglobin and myoglobin （Croci） were （6.42±1.51）μmol · L^-1 in the erector spinae, and （15. 48±4. 54）μmol ·L^-1 in the rectus femoris, respectively. The myoglobin was dominant in both of the results. These were consistent with the recent empirical reports. In summary, the NIRS method and prototype are authentic in detecting the pigment concentrations of pork tissue non-invasively, realime, directly and conveniently.

关 键 词：近红外光谱 色素 肌红蛋白 猪肉 浓度 

分 类 号：R318.5[医药卫生—生物医学工程]