多参数流式细胞术分析505例急性淋巴细胞白血病跨系列表达方式(英文)  

Cross-Lineage Expression in 505 Patients with Acute Lymphoblastic Leukemia by Multiparametric Flow Cytometry Analysis

作　　者：王兴兵[1] 杜雯[2] 夏亮[1] 郑金娥[2] 刘隽[2] 贺艳丽[2] 孙自敏[1] 黄士昂[2] 

机构地区：[1]安徽医科大学附属省立医院血液科,安徽合肥230001 [2]华中科技大学同济医学院附属协和医院干细胞研究与应用中心,湖北武汉430022

出　　处：《中国实验血液学杂志》2009年第6期1419-1423,共5页Journal of Experimental Hematology

基　　金：supported by the National Natural Science Foundation (30700329);Anhui Provincial Outstanding Young Investigator Program (08040106810)

摘　　要：跨系列抗原表达是白血病免疫表型的一个重要特征。对于急性淋巴细胞白血病(ALL)的跨系表达方式,目前国内尚缺乏大样本多参数的研究报道。本研究利用三色流式细胞术探讨505例ALL(431例B-ALL,74例T-ALL)患者23种系相关抗原的跨系表达方式。结果显示:全部ALL病例中,髓系抗原的表达率为56.4%,其中以CD13(32.7%)的表达最常见,其次为CD33(29.5%)、CD15(19.2%)和CD11b(7.7%)。CD13/CD33在CD34+病例中的表达高于CD34-病例。在B-ALL中,T系抗原CD4、CD5、CD7和CD2的表达率依次为6.3%、2.8%、1.9%和1.4%,并且CD7+、CD2+和CD4+病例通常共表达CD13和(或)CD33。在T-ALL,B系抗原cCD79a、CD19和CD22的表达率分别为8.1%、6.8%、和2.8%,而全部CD19和CD22表达者均伴CD13/CD33表达。结论:ALL的跨系表达多存在未成熟的阶段,以跨髓系抗原的表达最常见(B+M+,T+M+),偶有B系、T系和髓系抗原的共表达(B+T+M+),仅有B系和T系跨系表达的极少(B+T+M-)。The expression of immunological markers of one hematopoietic lineage on the abnormal cells of another lineage （ cross-lineage expression） is a known feature of leukemia. The present study was aimed to investigate the crosslineage expression in ALL cells. The cross-lineage expression in ALL cells from 505 patients was detected by flow cytometry using 23 monoctonal antibodies （McAbs） in triple staining combinations. The results showed that in whole ALL, the expression of myeloid antigens occurred in 56.4% of the cases, and CD13 was the most frequently expressed myeloid marker （32.7%） followed by CD33 （29.5%）, CD15 （19.2%） and CDllb （7.7%）. CD13/CD33 expres- sions were more frequent in CD34 ＋ cases than in CD34- cases. In B-ALL, T-cell antigen CD4, CD5, CD7 and CD2 were found in 27 （6.3%）, 12 （2.8%）, 8 （1.9%）, and 6 （1.4%） cases respectively, and CD7 ＋, CD2＋ and CD4＋ cases commonly expressed CD13/CD33. In T-ALL, B-cell antigen cCD79a, CD19 and CD22 were found in 6 （8.1%）, 5 （6.8%）, and 2 （2.8%） cases respectively, and all of CD19＋ and CD22＋ cases were all accompanied with CD13/CD33. It is concluded that cross-lineage expression in ALL mostly exists in the immature stages, ALL cells more frequently express phenotypes B ＋ M ＋ , T ＋ M ＋ and occasionally B ＋ T ＋ M ＋ , but B ＋ T ＋M - phenotype is extremely rare.

关 键 词：急性淋巴细胞白血病 免疫分型 跨系表达 

分 类 号：R733.7[医药卫生—肿瘤]