Cobetia marina CICC10367渗透压冲击下羟基四氢嘧啶的合成及释放  

Hydroxyectoine synthesis and release under osmotic shock in Cobetia marina CICC10367

作　　者：郎亚军[1] 任亚男[1] 柏林[1] 张苓花[1] 

机构地区：[1]大连海事大学环境科学与工程学院,大连116026

出　　处：《微生物学报》2009年第12期1590-1595,共6页Acta Microbiologica Sinica

基　　金：国家自然科学基金(20776021)~~

摘　　要：【目的】筛选获得耐受渗透压冲击的羟基四氢嘧啶合成菌株,利用"细菌挤奶"工艺提高羟基四氢嘧啶的产率。【方法】从盐池中分离羟基四氢嘧啶合成菌株,并对其进行形态、生理生化及16SrDNA鉴定。考察了培养基及其NaCl浓度对羟基四氢嘧啶合成的影响,在优化的条件下利用"细菌挤奶"工艺制备羟基四氢嘧啶。【结果】筛选获得的一株羟基四氢嘧啶合成菌株,鉴定为Cobetia marina CICC10367(C.marina CICC10367)。NaCl浓度为90g/L的、谷氨酸单钠为唯一碳氮源的培养基有利于羟基四氢嘧啶合成,最高合成量为694.5mg/L。6次渗透压冲击的细菌挤奶,羟基四氢嘧啶的总合成量4179.0mg/L,产率597.0mg/L/d,羟基四氢嘧啶对底物的转化率为80.2mg/g。【结论】筛选获得的菌株C.marina CICC10367在NaCl诱导下合成羟基四氢嘧啶,该菌株耐受渗透压冲击,利用细菌挤奶工艺显著地提高了该菌株羟基四氢嘧啶的产率和转化率。[ Objective] To obtain hydroxyectoine-producing strain with tolerance to osmotic shock and improve hydroxyectoine productivity by adopting ＂ bacteria milking＂ process. [ Methods] We isolated a strain from salt lake, and then carried out the identifications of morphology, physiological and biochemical characteristics of the strain. We also investigated the effects of the medium and its NaCl concentration on the hydroxyectoine synthesis of this strain. Under optimal condition, hydroxycctoine was produced by adopting ＂bacteria milking＂ process. [ Results] A hydroxyectoine-producing strain was obtained and identified as Cobetia marina CICC10367 （ C. marina CICC10367）. It could enhance hydroxyeceoine synthesis when the medium adopting monosodium glutamate as the sole source of carbon and nitrogen at 90 g/L NaCl. The highest synthesized hydroxyectoine concentration was 694.5 mg/L in the above described medium. After the ＂bacteria milking＂ process of six osmotic shock, the total concentration of hydroxyectoine was 4179.0 mg/L, the productivity was 597.0 mg/L/d, and the conversion rate of hydroxyectoine on substrate was 80.2 mg/g. [ Conclusion] C. marina CICC10367 was able to synthesize hydroxyectoine under NaCl induction and tolerant to osmotic stress. The hydroxyectoine productivity and conversion rate of hydroxyectoine on substrate were both significantly improved by adopting ＂bacteria milking＂ process.

关 键 词：Cobetia MARINA 羟基四氢嘧啶 渗透压冲击 细菌挤奶 

分 类 号：Q935[生物学—微生物学]