曲古菌素A对甲状腺癌细胞增殖及凋亡的影响  

Effects of Trichostatin A on Inhibiting Growth and Inducing Apoptosis of Thyroid Cancer Cells in Vitro

作　　者：林秀峰[1] 俞惠新[1] 包建东[1] 谭成[1] 张莉[1] 陈波[1] 

机构地区：[1]江苏省原子医学研究所卫生部核医学重点实验室,江苏无锡214063

出　　处：《肿瘤防治研究》2009年第12期999-1002,共4页Cancer Research on Prevention and Treatment

基　　金：江苏省卫生厅重点科研基金资助项目(H200623)

摘　　要：目的探讨组蛋白去乙酰化酶抑制剂曲古菌素A体外抑制甲状腺癌细胞增殖、诱导凋亡的作用。方法采用磺酰罗丹明B染色分析法、Hoechst33342/PI双染荧光显微镜检测及PI单染流式细胞仪分析等技术检测曲古菌素A对不同甲状腺癌细胞增殖、凋亡及细胞周期的影响。结果不同浓度的曲古菌素A处理48h,能明显抑制甲状腺癌细胞的增殖,并且随着药物浓度的增加抑制率也增大,各组间及与对照组相比差异均具有统计学意义(F=35.67,P<0.01);双染后镜下观察显示曲古菌素A使甲状腺癌细胞呈明显的凋亡状态,流式细胞仪分析则表明诱导后滤泡状甲状腺癌细胞凋亡峰(Sub-G1)比值升高,与对照组比较其差异有统计学意义(t=6.225,P<0.01)。结论曲古菌素A能显著抑制甲状腺癌细胞体外的增殖及诱导甲状腺癌细胞的凋亡,其作用呈浓度和时间依赖性。Objective To investigate the effects of Trichostatin A on inhibiting growth and induing apoptosis of thyroid cancer cell lines in vitro.Methods The cytotoxic effects of Trichostatin A on thyroid cancer cells were determined by using SRB assay.DNA content was measured by PI staining through flow cytometry.Apoptosis of thyroid cancer cells induced by Trichostatin A was identified by fluorescence microscopy with Hoechst33342/PI staining.Results After incubation of thyroid cancer cells with Trichostatin A for 48h, the inhibiting rate, with the augment of drug concentration, increased significantly（F = 35.67, P〈0. 01 ）. Trichostatin A induced apoptosis of thyroid cancer cells with Sub-G1 phase peak increasing in flow cytometry（t = 6. 225, P〈0. 01 ）. Conclusion Trichostatin A possesses inhibiting proliferation and apoptosis inducing activity on thyroid cancer cells. These effects appear to be dose-dependent.

关 键 词：曲古菌素A 甲状腺癌细胞 细胞增殖 细胞凋亡 

分 类 号：R736.1[医药卫生—肿瘤]