血管内皮生长因子受体3与PI3K/AKT在胃癌组织中的表达及意义  

Expression and Significance of Vascular Endothelial Growth Factor Receptor 3 and PI3K/Akt in Gastric Carcinoma Tissue

作　　者：苗莹莹[1] 朱正秋[2] 黄广清[2] 

机构地区：[1]徐州医学院研究生部,江苏徐州221002 [2]徐州医学院附属医院肿瘤内科

出　　处：《肿瘤防治研究》2009年第12期1039-1043,共5页Cancer Research on Prevention and Treatment

基　　金：徐州医学院资助课题(08KJ27)

摘　　要：目的观察血管内皮生长因子受体3(VEGFR-3)和磷脂酰肌醇-3-激酶(PI3K)、蛋白激酶B(PKB或Akt)在胃癌组织及相应癌旁组织中的表达情况。方法采用RT-PCR法检测36例胃癌及相应癌旁对照组织中VEGFR-3、PI3K、Akt mRNA的表达;免疫组织化学法检测VEGFR-3、P-Akt蛋白的表达,并用图像分析方法测定平均吸光度值。结果1.RT-PCR胃癌组织中VEGFR-3mRNA、PI3K mRNA、Akt mRNA与癌旁对照组织的表达水平相比,差异有统计学意义(P<0.05),且VEGFR-3mRNA与PI3KmRNA、Akt mRNA表达呈正相关(P<0.05)。VEGFR-3 mRNA表达与TNM分期、浸润深度、淋巴结转移与远处转移有关(P<0.05)。2.免疫组织化学VEGFR-3、P-Akt蛋白在胃癌组织中的表达水平明显高于癌旁对照组织(P<0.05),且VEGFR-3、P-Akt蛋白表达水平呈正相关(P<0.05)。结论VEGFR-3阳性表达的胃癌患者可能更易发生淋巴结转移,VEGFR-3可作为评估患者预后的重要指标。VEGFR-3与PI3K/Akt的表达具有一定的相关性。Objective To examine the expression of the vascular endothelial growth factor receptor 3,phosphatidylinositol-3-kinase（PI3K） and protein kinase B （ PKB or Akt） in gastric carcinoma and their adjacent normal tissues.Methods The mRNA expressions of VEGFR-3,PI3K and Akt in 36 cases of gastric carcinoma and their adjacent normal tissues were detected by reverse transcription-polymerase chain reaction （RT-PCR）.The protein expressions of VEGFR-3,P-Akt in the same tissues were detected by immunohistochemical method. Results 1. RT-PCR the mRNA expression levels of VEGFR-3, PI3K and Akt in gastric carcinoma tissues were higher than those of the adjacent normal tissues （P〈0. 05） . VEGFR-3 mRNA expression was correlated with PI3K mRNA and Akt mRNA expression（P〈 0. 05） . The mRNA expression level of VEGFR-3 was related TNM stage, depth of invasion, lymphnode metastasis and distant metastasis （P〈0. 05）. 2. Immunohistochemical the protein expression levels of VEGFR-3 and P-Akt in gastric carcinoma tissues were higher than those of the adjacent normal tissues （P〈0. 05）. VEGFR-3 protein expression was correlated with P-Akt protein expression （P〈0. 05） . Conclusion The tumors with VEGFR-3 expression are more likely to have lymphnode metastasis VEG- FR-3 is an important predictor for the prognosis of gastric carcinoma. The expression of VEGFR-3 and PBK/Akt have some relations.

关 键 词：血管内皮生长因子受体3 胃癌 磷脂酰肌醇-3-激酶 蛋白激酶B 淋巴结转移 

分 类 号：R735.2[医药卫生—肿瘤]