Caspase-3和hTERT在Barrett’s食管中的表达及其相互关系  

Expressions of Caspase-3 and hTERT and their relationship in Barrett's esophagus

作　　者：张汝钢[1] 高春芳[1] 房殿春[2] 王长松[1] 

机构地区：[1]解放军150医院消化内科,河南洛阳471031 [2]第三军医大学西南医院全军消化病研究所

出　　处：《胃肠病学和肝病学杂志》2009年第12期1099-1101,共3页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的研究半胱天冬蛋白酶-3(Caspase-3)和端粒酶逆转录酶(hTERT)在Barrett’s食管(Barrett’s esophagus,BE)及食管炎伴贲门腺或胃底腺化生组织中的表达,探讨其在BE发病中的作用。方法应用免疫组织化学法(LDP)检测22例BE和38例食管炎伴贲门腺或胃底腺化生组织中Caspase-3和hTERT的表达,比较两者表达的相关性。结果Caspase-3在BE中的阳性表达率为95.45%,明显高于在食管炎伴贲门腺或胃底腺化生组织中的表达(68.42%)(P=0.000);hTERT在BE中的阳性表达率为27.27%,明显低于在食管炎伴贲门腺或胃底腺化生组织中的表达(65.79%)(P=0.000)。BE中Caspase-3与hTERT的表达呈强负相关(r=-1,P=0.000)。结论BE中Caspase-3高表达,hTERT低表达。Caspase-3和hTERT在BE发生过程中起重要作用,可能成为预测BE发生的重要参考指标。Objective To study the expressions of Cycteine aspartate-specific protease-3 （Caspase-3） and human telomerase reverse transcriptase （hTERT） in Barrett＇ s esophagus （BE） and esophagitis with cardiac gland or fundus gland metaplasia, and to investigate their roles on pathogenesis of Barrett＇ s esophagus. Methods Immunochemical staining was used to detected the expressions of Caspase-3 and hTERT protein in 22 cases of BE and 38 cases of esophagitis with cardiac gland or fundns gland metaplasia. And their relationship was analyzed. Results The expression rate of Caspase-3 was significantly higher in BE （95.45%） than in esophagitis with cardiac gland or fundus gland metaplasia （68.42%） （P =0. 000）. However, the expression rate of hTERT was significantly lower in BE （27.27%） than in esophagitis with cardiac gland or fundus gland metaplasia （65.79%） （P = 0. 000）. The expression of Caspase-3 was negatively correlated with hTERT （r = - 1, P = 0. 000）. Conclusion Caspase-3 is overexpressed in BE, while hTERT is lowly expressed. Caspase-3 and hTERT might play important roles in the pathogenesis of BE. Detection of Caspase-3 and hTERT might be predictors in the prognosis of BE.

关 键 词：Barrett’s食管 半胱天冬蛋白酶-3 端粒酶逆转录酶 

分 类 号：R571[医药卫生—消化系统]