去甲肾上腺素和哌唑嗪对自发性高血压大鼠动脉平滑肌细胞膜Ca^(2+)-ATPase活性和PMCA1 mRNA表达的影响  

Effect of Norepinephrine and Prazosin on Ca^(2+)-ATPase Activity and mRNA Expression in Aortic Smooth Muscle Cells in Spontanously Hypertensive Rats

作　　者：姜黔峰[1,2] 商黔惠[1,2] 吴芹[3] 巩亮[1,2] 刘晓鹏[4] 

机构地区：[1]遵义医学院附属医院心内科 [2]遵义医学院临床医学研究所 [3]贵州省基础药理重点实验室,贵州省遵义市563003 [4]洛阳市中心医院急救中心,河南省洛阳市471000

出　　处：《中国动脉硬化杂志》2009年第10期815-818,共4页Chinese Journal of Arteriosclerosis

基　　金：贵州省2002年度科学技术基金重点资助项目(3013);贵州省优秀青年科技人才资助项目(0316)

摘　　要：目的观察去甲肾上腺素及其α1肾上腺素能受体拮抗剂哌唑嗪对自发性高血压大鼠血管平滑肌细胞Ca2+-ATPasee活性及mRNA表达的影响。方法采用生化酶学法和逆转录聚合酶链反应技术观察不同浓度去甲肾上腺素和哌唑嗪对自发性高血压大鼠血管平滑肌细胞Ca2+-ATPase活性和mRNA表达的影响。结果与自发性高血压大鼠组比较,10-7mol/L去甲肾上腺素能减弱Ca2+-ATPase活性(P<0.01)及mRNA的表达(P<0.01),10-5mol/L和10-6mol/L哌唑嗪能减弱10-7mol/L去甲肾上腺素对Ca2+-ATPase活性的抑制作用(P<0.05)。10-6mol/L哌唑嗪可抑制10-7mol/L去甲肾上腺素对PMCA1mRNA表达的影响(P<0.05)。单用哌唑嗪对Ca2+-ATPase活性及mRNA表达水平无明显改变(P>0.05)。结论去甲肾上腺素抑制自发性高血压大鼠Ca2+-ATPase活性和下调PMCA1mRNA表达,可能是通过α1肾上腺素能受体途径介导基因转录的下调。哌唑嗪可通过阻断α1肾上腺素能受体途径抑制去甲肾上腺素对自发性高血压大鼠血管平滑肌细胞Ca2+-ATPase活性和PMCA1mRNA表达的影响。Aim To observe the effects of norepinephrine （NE） and α1-adrenergic receptor antagonist prazosin on Ca^2＋-ATPase activity and mRNA expression of PMCA1 in cultured thoracic aortic smooth muscle cells （ASMC） isolated from spontaneously hypertensive rats （SHR）.Methods ATPase activity in cultured ASMC was determined by spectrophotometry and mRNA expression of PMCA1 was measured by semiquantitative reverse transcription polymerase chain reaction （RT-PCR）.The effects of different doses of NE and prazosin on ATPase activity and mRNA level of PMCA1 in ASMC were observed.Results NE（10^-7 mol/L）significantly attenuated the ATPase activity（P〈0.01） and mRNA expression of PMCA1（P〈0.01）.Compared with SHR control,10^-5 mol/L and 10^-6 mol/L prazosin attenuated the repressed effect of 10^-7 mol/L NE on Ca^2＋-ATPase activity （P〈0.05）.10^-6 mol/L prazosin could repress the effect of 10^-7 mol/L NE on mRNA expression of PMCA1（P〈0.05）.The ATPase activity and mRNA expression of PMCA1 in ASMC had no alteration after intervened by prazosin（P〉0.05）.Conclusions NE can attenuate the ATPase activity and mRNA expression of PMCA1 which may be through the pathway of α1-AR and mediate the downregulation of genetic transcription.Prazosin can inhibit the effect of NE on attenuating the Ca^2＋-ATPase activity and mRNA expression of SHR ASMC through blocking the α1-AR pathway.Prazosin had no effect on the activity and mRNA expression of PMCA1.

关 键 词：CA^2+-ATPASE 血管平滑肌细胞 去甲肾上腺素 哌唑嗪 自发性高血压大鼠 

分 类 号：R36[医药卫生—病理学]