线栓法制大鼠脑缺血模型操作和评价的改良  

Improvements in the establishment and evaluation of cerebral ischemia models made by thread occlusion in rats

作　　者：何斌[1] 张劲松[1] 邵斌霞[2] 程洁[3] 环飞[3] 肖杭[3] 高蓉[3] 鲁翔[4] 

机构地区：[1]南京医科大学第一附属医院急诊科,南京210029 [2]南京军区南京总医院 [3]南京医科大学毒理系神经毒理实验室 [4]南京医科大学第二附属医院

出　　处：《中华急诊医学杂志》2009年第12期1248-1251,共4页Chinese Journal of Emergency Medicine

基　　金：基金项目：江苏省自然科学基金资助项目（BS2006007）

摘　　要：目的 介绍线栓法制大鼠大脑中动脉栓塞模型的操作和评价的改良。方法雄性SD大鼠共120只，随机分成常规组（n=50）、改良组（n=60）和假手术组（n=10），前两组分别用常规线栓法和改良法制作大鼠脑缺血模型，利用体征指标和红四氮唑（2，3，5-triphenyltetrazolium chloride，TTC）染色对昕有大鼠进干亍评价？以TTC染色结果为金标准，比较两种造模方法的成功率，同时计算各项体征指标的灵敏度和特异度，率的比较用x^2或校正x^2检验。结果改良法模型成功率为71．67％，明显高于常规法的52．00％（P=0．034）；评价模型是否成功，现有的体征法灵敏度为98．55％，特异度仅为40．00％，经改良后灵敏度仍为98．55％，而特异度可提高到100．00％（P=0．000）。结论大鼠脑缺血模犁经改良后，造模成功率和评价准确性明显提高。Objective To modify the methods of operation and establishment of cerebral ischemia rat models made by thread occlusion. Method We randomly divided 120 male SD rats into a common group （ n = 50）, an improvement group （ n = 60） and a sham-operated group （ n = 10）. Rats in the common and improvement groups were made into models using the common and improvement methods separately. All models were evaluated on the basis of physical sign indices and 2,3,5-triphenyhetrazolkml chloride （TFC） staining. The TTC staining results were taken as gold standards. Then, we compared the achievement ratios of the two groups, and computed the sensitivity and specificity of every physical sign index based on these standards. The X^2 or correction X^2 test was used to compare the ratios. Results （ 1 ） The achievement ratio in the improvement group was significantly higher than that in the common group （71.67% vs. 52.00%, P = 0.034）. （2） The sensitivity of evaluation for both common and improvement methods was 98.55%. However, the specificity of evaluation for the improvement method was significantly higher than that for the common method （100.00% vs. 40.00%, P = 0.000）. Conclusions The establishment achievement ratio and evaluation correctness of models are obviously elevated by modification of the operation and establishment methods.

关 键 词：脑缺血 大鼠 动物模型 模型制作 模型评价 成功率 组织染色 神经功能评分 

分 类 号：R-332[医药卫生]