鸡白细胞介素-18真核表达载体构建与ILTV弱毒疫苗联合免疫  

Construction of Eukaryotic Expression Vector of Chicken Interleukin IL-18 and Its Combined Immunity with ILTV Attenuated Vaccine

作　　者：邵攀峰[1] 陈红英[1] 崔保安[1] 王振亚[1] 宋亚鹏[1] 管倩[1,2] 刘金朋[1] 

机构地区：[1]河南农业大学牧医工程学院,郑州450002 [2]河南牧翔动物药业有限公司,郑州450008

出　　处：《四川农业大学学报》2009年第4期487-492,共6页Journal of Sichuan Agricultural University

基　　金：国家"十一五"科技支撑计划专项(2006BAD06A08)

摘　　要：为了探索鸡IL-18在传染性喉气管炎病毒(ILTV)弱毒疫苗中的免疫佐剂作用,将鸡IL-18(ChIL-18)克隆至真核表达载体pcDNA3.1(+)中,构建重组真核表达质粒pcDNA3.1/ChIL-18(简称pIL-18)。用pIL-18、ILTV弱毒疫苗及其两者联合分别免疫21日龄SPF雏鸡,免疫后每周抽取外周血及外周抗凝血,应用ELISA和流式细胞仪分别检测免疫鸡的体液免疫及细胞免疫水平。免疫后第4周用100 ELD50ILTV强毒进行攻击。结果pIL-18与ILTV弱毒疫苗联合免疫组的淋巴细胞亚群CD3+、CD3+CD4+、CD3+CD8+明显高于ILTV弱毒疫苗免疫组(P<0.01),ILTV强毒攻毒后发病鸡数量少,并且发病症状较轻。To study the adjuvant effect of chicken interleukin-18 （ChIL-18） on Infectious Laryngotracheitis Virus （ILTV） attenuated vaccine, chicken IL-18 gene was cloned and transformed into eukaryotic expressing vector pcDNA3.1 （＋） to generate an expressing plasmid pcDNA3.1/ChIL- 18 （pIL-18）. The 21-day-old SPF chickens were immunized with pIL-18, ILTV attenuated vaccine and combined immunity with both of them respectively. After that, peripheral blood and peripheral anti-coagulation were detected by flow cytometry and ELISA method at humeral immunity and cellular immunity level each week, respectively. Virulence 100 ELD50 ILTV was given to the chickens at 4 weeks after vaccination. The results showed that chickens vaccinated with combined immunity of pIL-18 and ILTV attenuated vaccine had an significancy increase in the percentage of CD3^＋ , CD3^＋CD4^＋ , and CD3^＋CD8^＋ subgroups of peripheral blood compared with that only immunized by ILTV attenuated vaccine. Chickens were given virulence to. have fewer diseases and milder symptom than others.

关 键 词：鸡 白细胞介素-18 真核表达载体 传染性喉气管炎病毒 疫苗 

分 类 号：S852.4[农业科学—基础兽医学]