免疫毒素Luffin B-Ng76对人黑色素瘤细胞的体外抑制作用  

In Vitro Inhibition of Human Melanoma Cells by Immunotoxin Luffin B Ng76

作　　者：张茹平[1] 熊长云[1] 严明[1] 张祖传[1] 

机构地区：[1]中国科学院上海生物化学研究所

出　　处：《生物化学与生物物理学报》1998年第6期561-564,共4页

摘　　要：用ＢｌｕｅＳｅｐｈａｒｏｓｅＣＬ－６Ｂ凝胶亲和层析法从丝瓜籽中分离纯化了单链致核糖体失活蛋白（ｒｉｂｏｓｏｍｅｉｎａｃ－ｔｉｖａｔｉｎｇｐｒｏｔｅｉｎ，ＲＩＰ）——ｌｕｆｉｎＢ。并将ｌｕｆｉｎＢ与抗人黑色素瘤细胞单抗Ｎｇ７６制成了免疫毒素，命名为ｌｕｆｉｎＢ－Ｎｇ７６，它对体外培养的黑色素瘤细胞Ｍ２１有很强的抑制作用，ＩＣ５０为２．５×１０－１１ｍｏｌ／Ｌ，毒性比游离的ｌｕｆｉｎＢ提高４０００倍，而它对非靶的ＨｅＬａ细胞的毒性较Ｍ２１细胞低１２００倍。结果提示ｌｕｆｉｎＢ用于制备免疫毒素具有良好的应用前景。Luffin B, a plant single chain ribosome inactivating protein, was purified from seeds of Luffa cylindrica by Blue Sepharose CL 6B affinity chromatography. An immunotoxin was constructed with luffin B and Ng76, a monoclonal antibody to human melanoma cell M 21 . Luffin B Ng76 showed 4 000 fold more cytotoxic to target melanoma cells than free luffin B. The IC 50 of luffin B Ng76 for M 21 cells and non target HeLa cells was 2.5×10 -11 mol/L and 3.0×10 -8 mol/L, respectively. The results suggest that luffin B is a new potent immunotoxin effector.

关 键 词：免疫毒素 人黑色素瘤细胞 体外抑制作用 

分 类 号：R739.5[医药卫生—肿瘤]