马铃薯GBSS基因5′侧翼区调控作用的研究  

STUDY ON REGULATION OF 5′ FLANKING REGIONS OF GRANULE BOUND STARCH SYNTHASE GENE OF POTATO 

作　　者：宋东光[1] 孙国枫[1] 单海燕[1] 彭翔寅 王光清[1] 汪训明[1] 谈家桢[1] 

机构地区：[1]复旦大学遗传学研究所遗传工程国家重点实验室

出　　处：《Acta Botanica Sinica》1998年第9期796-802,共7页Acta Botanica Sinica（植物学报：英文版）

摘　　要：将０．４、０．８、１．６、２．９ｋｂＧＢＳＳ基因的５′侧翼区与ＧＵＳ基因融合，构建了双元表达载体。０．８ｋｂＧＢＳＳＧＵＳ通过基因枪介导在块茎切片中获得了瞬间表达。以上建构物通过农杆菌介导转入了马铃薯（ＳｏｌａｎｕｍｔｕｂｅｒｏｓｕｍＬ．ｃｖ．Ｄｅｓｉｒｅｅ）。ＸＧｌｕｃ染色及ＰＣＲ结果证实已获得转基因植株。利用离体块茎诱导系统，ＧＵＳ表达用荧光进行定量检测，结果显示，２．９、１．６、０．４ｋｂＧＢＳＳＧＵＳ的表达均以块茎明显高于茎段，达２～１０倍。０．８、１．６、２．９ｋｂＧＢＳＳＧＵＳ表达高于０．４ｋｂＧＢＳＳＧＵＳ。蔗糖浓度的升高可诱导ＧＢＳＳＧＵＳ的表达，而光照抑制了ＧＢＳＳＧＵＳ的表达。Binary vectors were constructed by fusing 0.4, 0.8, 1.6, and 2.9 kb 5′ flanking regions of GBSS gene with GUS (β glucuronidase). Transient GUS expression was observed in in vitro tuber slices bombarded with 0.8 kb GBSS GUS construct. These constructs were then transferred into potato ( Solanum tuberosum L. cv. Desiree) via Agrobacterium tumefaciens transformation. Transgenic potato plants were confirmed by X Gluc staining and PCR. Using in vitro tuberization system, GUS expressions were assayed with fluorescence, it was shown that 0.8, 1.6 and 2.9 kb GBSS GUS expressions were higher than 0.4 kb GBSS GUS. 1.6 and 2.9 kb GBSS GUS expressions were about 2 to 10 folds higher in tubers than in stems. In cultured shoots, GBSS GUS expression could be induced by increased sucrose concentration but inhibited by light.

关 键 词：马铃薯 基因调控 GBSS基因 块茎专一表达 诱导 

分 类 号：Q756[生物学—分子生物学] S532.032[农业科学—作物学]