GC32细胞对三种虫媒病毒的敏感性研究  

Susceptibility of GC32 Cell to Three Strains of Arbovirus

作　　者：章域震[1] 黄文丽[1] 侯宗柳[1] 袁庆虹[1] 

机构地区：[1]云南省流行病防治研究所

出　　处：《中国病毒学》1998年第3期222-225,共4页Virologica Sinica

基　　金：云南省卫生厅科研基金

摘　　要：用胃癌细胞株ＧＣ３２，对乙型脑炎、基孔肯雅、兰加特病毒进行敏感性研究。并用ＢＨＫ２１细胞作对照，发现ＧＣ３２细胞对两种病毒的敏感性与对照细胞ＢＨＫ２１极为接近。ＢＨＫ２１和ＧＣ３２细胞对基孔肯雅、乙型脑炎和兰加特的免疫荧光实验，于感染后４８ｈ或７２ｈ都出现＋＋＋～＋＋＋＋的阳性结果。两种细胞对基孔肯雅和乙型脑炎病毒都形成空斑，ＢＨＫ２１细胞对基孔肯雅和乙型脑炎病毒的空斑形成单位分别为５．６５和５．３６；ＧＣ３２对基孔肯雅和乙型脑炎病毒的空斑形成单位分别为６．４８和５．６１。实验表明，ＧＣ３２细胞可以作为有关病毒实验的理想细胞株。GC32 cell line established by authors from a carcinoma patient is susceptible to JE and Chikungunya. The susceptibility of GC32 cell to JE and Chikungunya is similar to that of BHK21 regarded as control. BHK21 and GC32 cells infected by JE, Chikungunya and Langat viruses make “ +++～++++ ” results by IFAT. Both BHK21 and GC32 cells to JE and Chikungunya form plaques.The PFU of BHK21 cell to JE and Chikungunya are 5.36 and 5.65 and GC32 cell to JE and Chikungunya are 5.61 and 6.48, respectively. The results showed that GC32 cell line is a suitable cell strain for studying virus.

关 键 词：GC32细胞 敏感性 空斑形成单位 免疫荧光实验 

分 类 号：R735.2[医药卫生—肿瘤] R373.3[医药卫生—临床医学]