微波诱变和激光诱变相结合选育金霉素链霉菌的研究  

Studies on the Screening of High Yielding Demethylchlorotetracycline Strain by Combining Laser Irradiation with Microwave Irradiation

作　　者：李永泉[1] 贺筱蓉[1] 

机构地区：[1]浙江大学生命科学学院

出　　处：《生物工程学报》1998年第4期445-448,共4页Chinese Journal of Biotechnology

摘　　要：采用激光和微波两种物理因子，对去甲基金霉素生产菌金霉素链霉菌进行诱变处理，选育到一株产量较高的生产菌ＨＬ１１，发酵效价从２８３１ｕ／ｍｌ提高到４６８３ｕ／ｍｌ，提高了６５４％。所选育的菌株经多次传代，遗传性状非常稳定。A Mutant strain HL11 of high yielding demethychlorotelracycline was screened form its parent strain S.aureofaciens MHO1 by combining laser irradiation with microwave irradiation.The results showed that the titer of demethylchlorotetracycline of S.aureofaciens was increased from 2831 u/ml upto 4683 u/ml and the increasing rate was 65.4%.It's high capacity for producing demethychorotetracycline remained stable after many times of subculture and two months storage.

关 键 词：激光诱变 微波诱变 金霉素链霉菌 去甲基金霉素 

分 类 号：Q939.97[生物学—微生物学] TQ920.1[轻工技术与工程—发酵工程]