梭形住肉孢子虫和巨型住肉孢子虫包囊各组份抗原特性研究  

ANTIGENICITY OF VARIOUS FRACTIONS OF SARCOCYSTIS FUSIFORMIS IN BUFFALO AND SARCOCYSTIS GIGANTEA IN SHEEP

作　　者：肖兵南[1,2] 张长弓[1,2] 周望平 曾宪芳[1,2] 易新元 

机构地区：[1]湖南省畜牧兽医研究所 [2]湖南医科大学

出　　处：《寄生虫与医学昆虫学报》1998年第3期136-141,共6页Acta Parasitologica et Medica Entomologica Sinica

摘　　要：本文对采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（ＳＤＳ—ＰＡＧＥ）对梭形住肉孢子虫和巨型住肉孢子虫的包囊壁（ＣＷ）、包囊液（ＣＦ）与缓殖子（ＣＴ）三部分的可溶性蛋白进行了分析，从各组份区分出１０—２６条蛋白带，分子量９—１００ｋＤａ，ＣＷ以高分子量蛋白为主，ＣＦ则以５０ｋＤａ以下低分子量蛋白带居多，ＣＴ各种蛋白带分布较均匀。应用超凝胶ＡＣＡ５４柱层析和ＳＤＳ—ＰＡＧＥ对梭形住肉孢子虫包囊蛋白进行了分离纯化，分离出７２ｋＤａ蛋白，经免疫印渍术鉴定为单特异性抗原、经对８６头水牛的ＥＬＩＳＡ检疫分析，其纯化抗原较未纯化的粗抗原，特异性增强，但敏感性降低。Soluble antigens extracted from cyst wall(CW),cysttozoites (CT) and cyst fluid(CF) of Sarcocystis fusiformis and S.gigantea from naturally infected animals were analysed for the molecular weights and immunological characteristics by SDS—PAGE.Western blot and ELISA.Bands from 10 to 26 with Mwranging from approximately 9 to 100kDa were separated from various antigenic extracts.Antigens from CW and CT are mainly larger molecular proteins,while the antigens from CF mainly consist of smaller molecular proteins under 50kDa.ACA54 ultragel chromatography was adopted to isolate and purify specific antigens from crude antigen preparation of S.fusiformis cysts.72kDa protein from purified antigens and 175,53,72,80,90,and 100kDa proteins from whole cyst extracts were identified by SDS—PAGE and Western blot.It has been demonstrated by ELISA that the purified antigen had lower sensitivity and higher specificity in comparison with the crude antigens.

关 键 词：住肉孢子虫 抗原性 SDS-PAGE 免疫印渍 

分 类 号：R382.34[医药卫生—医学寄生虫学]