多能硫杆菌RuhisCO墓因在大肠杆菌中表达产物分析  

ANALYSIS OF EXPRESSING PRODUCTS OF RUBISCO FROM THIOBACILLUS VERSUTUS IN ESCHERICHIA COLI

作　　者：刘振盈[1] 张华[2] 

机构地区：[1]山东大学微生物技术国家重点实验室,济南250100 [2]山东中医药大学,济南250014

出　　处：《微生物学通报》1998年第4期199-201,共3页Microbiology China

摘　　要：通过对多能硫杆菌RubiSCO的基因表达分析表明该基因能够在pBR322的P1启动子、pUC19的lac启动子以及pKK223-3的tac启动子的启动下，在大肠杆菌中表达，RubisCO基因片段在pUC19和pKK223-3载体上的表达活性较高。进一步对RubisCO基因表达产物进行了非变性聚丙烯酰胺凝胶电泳，检测到了RubisCO蛋白质带。The Rubis CO gene of T. versutus has been successfully expressed in E. colt underthe P1(pBR322), lac(pUC19) and tac (pkk223-3) promoters. ms fragment showed high levelastivities in pUC19 and pKK23-3. Nondenaturing PAGE was performed with each crudeextract of E colt JM109 containing RubisCO gene at various vectors. The product of theexpression of T versutus RubisCO gene have been observed on PAGE electrophoresis.

关 键 词：多能硫杆菌 RUBISCO基因 大肠杆菌 表达 

分 类 号：Q78[生物学—分子生物学] Q933