白细胞介素-1α及IL-1RA与多发性硬化关系的研究  

The relationship between interleukin-1α and interleukin-1 receptor antagonist (IL-1RA) gene polymorphism and the multiple sclerosis patients in the Northeast of China

作　　者：毕胜[1] 韩丽丽[2] 潘尚哈[1] 王雪婷[2] 王振峰[2] 隋丹[2] 王真奎[2] 赵维纳[2] 李毅[2] 任亚静[2] 刘国军[2] 巩凤超[2] 钟华[2] 

机构地区：[1]哈尔滨医科大学第一临床医学院中心实验室,黑龙江哈尔滨150001 [2]哈尔滨医科大学第一临床医学院神经内科,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2009年第21期4030-4032,共3页Progress in Modern Biomedicine

基　　金：黑龙江省政府博士后启动金资助项目(编号:LBH-Q04071)

摘　　要：目的:探讨白细胞介素-1(interleukin-1,IL-1),尤其是白细胞介素-1α(IL-1α)和白细胞介素-1受体拮抗剂(IL-1RA)基因多态性与东北地区汉族人群多发性硬化(multiple sclerosis,MS)的关系。方法:多聚酶链反应(PCR)进行IL-1α和IL-1RA基因多态性检测。结果:IL-1α和IL-1RA基因型分布及等位基因频率在MS和对照组间无显著性差异(P>0.05)。结论:IL-1α和IL-1RA基因多态性与MS易感性无相关性。Objective：To explore the relationship between interleukin-1,especially interleukin-1α（IL-1α） and interleukin-1 receptor antagonist（IL-1RA） gene polymorphism and the multiple sclerosis（MS） patients in the Northeast of China. Methods：DNA of white blood cells from 54 subjects with MS and 60 healthy controls was extracted. Polymorphisms in IL-1α and IL-1RA were analyzed with PCR in order to find the difference of the genotype distribution and the allele frequency between the MS patients and the healthy controls. Results：The genotype（CC,CT,TT） distribution of IL-1 α was 40.7%,24.1%,and35.2% in patients with MS and 43.4%,23.3%,and 33.3% in controls（P〉0.05） ;while for the IL-1RA（I/I,I/II,II/II,I/IV） was81.5%,7.4%,5.6%,5.5% in MS patients and 80.0%,8.3%,5.0%,6.7% in controls（P〉0.05） . The allele frequency of IL-1α in patients with MS were C 57.4%,T42.6% and 60.0%,40.0% in controls;while for the IL-1RA（I,II,IV） were 85.2%,9.3%,5.5% in MS patients and 83.3%,10.0%,6.7% in controls.（P〉0.05） . Conclusion：The IL-1α and IL-1RA polymorphisms were not significantly associated with the susceptibility of MS.

关 键 词：IL-1Α IL-1RA 多发性硬化 基因多态性 

分 类 号：R744.51[医药卫生—神经病学与精神病学]