稀有人参皂苷IH901酶法转化与制备研究  

Study on Enzymatic Transformation and Preparation of Rare Ginsenoside IH901

作　　者：童庆宣[1] 陈良华[1] 明艳林[1] 郑国华[1] 齐晓辉[1] 郑志忠[1] 

机构地区：[1]厦门华侨亚热带植物引种园天然产物研发中心,厦门361002

出　　处：《天然产物研究与开发》2009年第6期1039-1044,共6页Natural Product Research and Development

基　　金：厦门市科技创新资金项目(3502Z20062008;3502Z20061143)

摘　　要：本研究利用酶制剂蜗牛酶,酶法转化三七二醇组皂苷制备稀有人参皂苷IH901,正交实验优化酶解条件,建立酶法转化工艺。结果表明:超声法提取三七总皂苷正交实验优化条件为用75%乙醇溶液,15倍溶剂用量,超声波提取210min作为最佳条件,三七总皂苷得率为12.21%;酶法转化二醇组人参皂苷制备稀有人参皂苷IH901,正交实验优化的条件为物料比为6/1、反应时间9h、反应温度为45℃、pH值为3.0,酶解得率为54.24%;经硅胶柱分离获得IH901单体化合物,HPLC测定纯度达98%。酶法转化制备皂苷IH901的工艺方法简便,切实可行,可为中试生产提供参考。Rare ginsenoside IH901 was preparated through enzymatic transformation from protopanaxadiol ginsenoside of Partax notoginseng by snailase, hydrolysis conditions were optimized by the orthogonal test, the enzymatic conversion process was estabilished. The results showed that orthogonal test for optimization conditions of Ultrasonic extraction of Panax notoginoside （PNS） was solvent for 75% ethanol solution, 15 times the amount of solvent,210 rain ultrasonie extraction as the best optimization of conditions ,PNS was 12.21 percent rate; the affecting factors of enzymatic transformation were studied and optimized, and the best optimal conditions were obtained as following ：6/1 for material ratio, 9 h for reaction time ,45 ℃ as reaetion temperature and 3.0 as pH value,the optimum rate of etude hydrolysate was 54.24%. The ginsenoside IH901 was purified by silica gel column ehromatogram, its purity was about 98% analyzed by HPLC. Enzymatic conversion process of IH901 preparation is simple,practical, can provide a reference in the trial production.

关 键 词：二醇组人参皂苷 酶法转化 稀有人参皂苷IH901 

分 类 号：Q946.91[生物学—植物学] R284.2[医药卫生—中药学]