凉血化瘀方对实验性脉络膜新生血管VEGF与PEDF表达的影响  

Decoction of Liangxue Huayu on expression of VEGF and PEDF in experimental choroidal neovascularization

作　　者：唐由之[1] 王慧娟[1] 冯俊[1] 

机构地区：[1]中国中医科学院眼科医院,北京市100040

出　　处：《眼科新进展》2009年第12期881-885,共5页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:30472229)~~

摘　　要：目的观察凉血化瘀方对氪激光诱导的实验性脉络膜新生血管(choroidal neovascularization,CNV)中新生血管内皮生成因子(vascular endothelial growth factor,VEGF)和色素上皮衍生因子(pigment epithelium derived factor,PEDF)水平的影响。方法BN大鼠32只,随机分为对照组及凉血化瘀方组(中药组),每组大鼠各16只。采用氪激光击破大鼠Bruch膜的方法诱导实验性CNV BN大鼠。观察激光光凝后1周、2周、3周、4周时2组大鼠眼底CNV形成情况,采用免疫组织化学法测量VEGF和PEDF的表达水平。结果光凝后2组均有CNV形成。中药组大鼠在2-4周时CNV荧光素渗漏面积较对照组小,差异均有统计学意义(均为P<0.05)。免疫组织化学结果示对照组VEGF表达在4周时达到高峰,积分光密度(integral optical density,IOD)均值为1552.66±753.50,中药组在2周时达到高峰,IOD均值为212.83±160.20,3周时表达下调,光凝后2-4周,2组VEGF光密度值差异均有统计学意义(均为P<0.05);对照组和中药组的PEDF在1周时表达水平最高,IOD均值分别为162.42±45.58和493.85±127.35,此后2组表达水平均呈下降趋势,光凝后1-4周,2组间PEDF IOD均值差异均有统计学意义(均为P<0.05)。结论凉血化瘀方中药抑制实验性CNV生长的作用可能是通过下调VEGF表达和上调PEDF表达而实现的。Objective To investigate the effects of decoction of Liangxue Huayu on the expression of vascular endothelial growth factor（VEGF）and pigment epithelium derived factor（PEDF）in krypton laser induced experimental choroidal neovascularization（CNV）.Methods A total of 32 Brown Norway rats were randomly divided into control group and decoction of Liangxue Huayu group（traditional Chinese medicine group）,16 rats in each group.Experimental CNV Brown Norway rats were induced by krypton laser to break Bruch membrane of rats. The formation of CNV at fundus were observed and the expression of VEGF and PEDF were measured with immunohisto- chemistry at 1 week,2 weeks,3 weeks and 4 weeks after laser photocoagulation in both two groups. Resultu The formation of CNV was found in both two groups after laser photocoagulation. Fluorescein leakage area of CNV was fewer in traditional Chinese medicine group than that in control group at 2 weeks to 4 weeks after laser photocoagulation（ all P 〈 0.05 ）. Immunohistochemistry showed that the expression of VEGF came to the peak at 4 weeks and the average integral optical density （IOD） value was 1 552. 66± 753. 50 in control group; The expression of VEGF came to the peak at 2 weeks,the average IOD value was 212.83± 160.20 and began to decrease at 3 weeks in traditional Chinese medicine group. At 2 weeks to 4 weeks after photocoagulation, the average IOD value of VEGF had statistical difference between the two groups （ all P 〈 0. 05）. The expression of PEDF reached to the most at 1 week in both groups, were 162. 42 ± 45.58 in control group and 493. 85 ± 127. 35 in traditional Chinese medicine grouo：There was a downtrend of PEDF exoression in both two grouos in the following time. At 1 week to 4 weeks after photocoagulation, there was statistical difference in average IOD value of PEDF between the two groups （ all P 〈 0.05 ）. Conclusion Decoction of Liangxue Huayu can inhibit the growth of experimental CNV through the way of down-regulating the level

关 键 词：凉血化瘀方 脉络膜新生血管 血管内皮生长因子 色素上皮衍生因子 

分 类 号：Q959.836[生物学—动物学] R773.4[医药卫生—眼科]