40例非梗阻性无精子症患者的细胞遗传学和分子遗传学研究  

40 azoospermia patients cytogenetics and molecular genetics research

作　　者：陈桂兰[1] 钟永红[1] 许红雁[1] 蔡珠[1] 李小红[1] 黎建欣[1] 

机构地区：[1]广东粤北人民医院,广东韶关512026

出　　处：《中国民康医学》2010年第1期3-4,6,共3页Medical Journal of Chinese People’s Health

摘　　要：目的:探讨非梗阻性无精子症的细胞和分子遗传学特点及其对生殖的影响,为非梗阻性无精子症患者的治疗提供理论依据方法:采用外周血染色体G显带和多重聚合酶链反应技术等方法进行染色体分析及AZF基因的15个位点进行分析,正常对照组为已经生育的男性。结果:40例非梗阻性无精子症患者中检测出8例染色体异常,6例AZF基因微缺失。结论:非梗阻性无精子症与遗传密切相关,对非梗阻性无精子症患者进行细胞遗传学及Y染色体AZF区域微缺失检查是必要的。Objective： To investigate the genetic characters of azoospermia patients and its influence on male reproduction. Methods：Blood samples were obtained from 40 azoospermia male patients. The circumference blood chromosome G obviously techniques were used for the chromosome analysis. The AZF gene 15 unit＇s places pots were analyzed by multiple polymerase chain reactions. Results： In 40 detected examples, there are 8 examples which are detected abnormal karyotypes, 6 samples that have AZF gene micro flaw. Conclusions： Azoospermia are highly relevant with genetic abnormalities. It is necessary to carry on chromosome analysis and detect the Y chromosome AZF gene micro flaw in infertile male patients.

关 键 词：男性不育 无精子症 染色体核型分析 AZF基因 

分 类 号：R711.6[医药卫生—妇产科学]