病毒感染和饥饿对不同细胞GCM1和syncytin 1表达的影响  

Effects of Viral Infection and Starvation on the Transcription of GCM1 and Syncytin 1 in Different Cells

作　　者：姚源蓉[1] 

机构地区：[1]天津医科大学总医院神经内科,天津市神经病学研究所,300052

出　　处：《天津医药》2009年第12期996-999,共4页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:30800356);天津市自然科学基金资助项目(项目编号:07JCYBJC16200);教育部留学回国人员科研启动基金资助项目(项目编号:2008第890号);天津医科大学总医院引进人才科研启动基金

摘　　要：目的:研究病毒感染和饥饿外环境因子对不同细胞系syncytin 1和转录因子glialcell missing 1(GCM1)表达的影响,进一步探讨GCM1在不同细胞系对syncytin 1转录的调控作用。方法:应用实时RT-PCR检测细胞GCM1和syncytin 1 mRNA水平,并采用RNA干扰技术抑制GCM1的转录,进一步探讨GCM1是否直接调控syncytin 1在星形胶质细胞中的表达。结果:病毒感染和饥饿处理使不同细胞的GCM1和syncytin 1转录水平不同程度增高。RNA干扰使绒癌细胞系JEG-3细胞的GCM1 mRNA水平比对照组明显降低(P<0.05),同时syncytin 1的mRNA水平也较对照组降低(P<0.01)。然而,RNA干扰使受病毒感染或饥饿处理的人星形胶质细胞瘤细胞CCF-STTG1的GCM1 mRNA水平明显降低后,syncytin 1 mRNA水平与病毒感染或饥饿处理组比较差异无统计学意义。结论:病毒感染和饥饿外环境因子可上调细胞GCM1和sycnytin 1的转录,GCM1不直接调控syncytin 1在星形胶质细胞的表达。Objective：To investigate the effects of viral infection and starvation on the transcription of syncytin 1 and glial cell missing 1 （GCM1）, and the modulation of GCM1 on the transcription of syncytin 1 in different cells thereof. Methods： The mRNA levels of GCM1 and syncytin 1 were measured with real time RT-PCR. Furthermore, the possible modulation of GCM1 on the transcription of syncytin 1 was explored in astrocytes by RNA interference against GCM1.Results：The transcrip- tional levels of GCM1 and syncytin 1 were up-regulated in different cells at various levels under the stimulation of both viral infection and starvation. Accompany with the reduction of GCM1 mRNA levels （P 0.05, as compared to controls） induced by GCM1 RNA interference, the mRNA level of syncytin 1 reduced significantly in JEG-3 cells compared to that of controls （P 0.01）. However, the syncytin 1 mRNA level had no obvious change in CCF-STTG1 cells notwithstanding the significant reduc- tion of GCM1 mRNA level induced by GCM1 RNA interference. Conclusion： This study suggests that viral infection and star- vation could up-regulate the transcription of GCM1 and syncytin 1, and GCM1 may not directly modulate the transcription of syncytin 1 in astrocytes.

关 键 词：病毒 感染 饥饿 基因表达调控 转录因子 细胞系 肿瘤 星形细胞 RNA干扰 

分 类 号：R73-36[医药卫生—肿瘤] R512.62[医药卫生—临床医学]