胃癌及癌前病变中的TFF-1、EGFR表达与血管再生的相关性研究  

Expression of TFF-1,EGFR in Gastric Carcinoma and Preacancenous Disease and its Relationship with Angiogeneisis

作　　者：潘卫新[1] 卜平[1] 朱海航[2] 孔桂美[1] 殷刚峰[1] 李国利[1] 

机构地区：[1]扬州大学医学院消化病研究所,扬州225001 [2]苏北人民医院

出　　处：《临床消化病杂志》2009年第6期339-341,共3页Chinese Journal of Clinical Gastroenterology

基　　金：国家自然科学基金资助(30873343/C190402)

摘　　要：目的探讨胃癌及癌前病变中三叶因子-1(trefoil factor1,TFF-1)、表皮生长因子受体(epidermal growthfactor receptor,EGFR)与血管形成的关系。方法将121例患者存档标本分为萎缩性胃炎伴肠化生、不典型增生、高分化胃癌和低分化胃癌4组,另取29例体检正常人标本作为正常组。采用免疫组化SP方法测定TFF-1、EGFR值;同时所有对象用抗CD34血管标记物表达测微血管密度(microvessel density,MDV)值,并进行相关性分析。结果在正常对照组及萎缩性胃炎伴肠化生、不典型增生、高分化胃癌和低分化胃癌各组中,TFF-1阳性表达率分别为100.0%、85.18%、81.82%、64.00%、38.39%,呈逐渐减弱趋势。后4组与正常组比较有显著差异,高分化胃癌组表达高于低分化胃癌组(P<0.05);而EGFR在正常组、萎缩性胃炎伴肠化生、不典型增生、高分化胃癌和低分化胃癌组中的表达分别为24.13%、40.74%、42.42%、56.00%、72.22%,呈逐渐增加趋势。后4组与正常组比较有显著差异,高分化胃癌组表达低于低分胃癌组(P<0.05)。结论实验结果显示TFF-1与EGFR呈负相关(r=-0.913,P<0.01)。EGFR值与MDV呈正相关(r=0.936,P<0.01)。TFF-1与EGFR在胃癌的早期诊断中起重要作用,可以作为判断肿瘤转移与否及手术前肿瘤是否复发的评判标准。Objective Investigate trefoil factor-1 （TFF-1） ,EGFR in precanerous condition and gastric cancer and its relationship to gastric cancer angiogenesiso Methods 121 cases atrophic gastritis with intestinal metaplasia,atypical hyperplasia, well-differentiated gastric carcinoma and poorly differentiated gastric cancer were divided into 4 group. Another 29 cases with normal gastric mucosa as the control group. Using immunohistochemical SP method to determine TFF-1, EGFR value. All subjects were measured the expression of vascular markers of microvessel density （MDV） value with anti-CD 34,and correlation was analysed. Results In the normal gastric mucosa group, atrophic gastritis with intestinal metaplasia group, atypical hyperplasia group,well-differentiated and poorly differentiated gastric cancer group,TFF-1 positive expression rates were 100. % ,85. 18%, 81.82% ,64. 00% ,38. 89%, the trend was gradually weakened. Well-differentiated gastric carncer group were significantly different than in poorly differentiated gastric cancer group（ P 〈 0. 05 ）. And EGFR in the normal group,atrophic gastritis with intestinal metaplasia group, atypical hyperplasia group, well-differentiated and poorly differentiated gastric cancer group were 24. 13% , 40. 74% ,56.00% ,72.22% ,showing a gradual increase in the trend, normal group compare with others were significantly different,well-differentiated gastric cancer group was weaker than in poorly differentiated gastric cancer group（ P 〈0. 05 ）. Conclusion Experimental results showed that EGFR and TFF-1 negatively correlated（ r = - 0. 916, P 〈 0. 01 ）. TFF-1 and EGFR play an important role in the early diagnosis of gastric cancer,which can be used as turner metastasis and to determine whether is pre-operative relapse criteria.

关 键 词：胃癌 胃癌前病变 三叶因子-1 表皮生长因子受体 微血管密度 

分 类 号：R735.2[医药卫生—肿瘤]