人结肠癌细胞系肿瘤干细胞特性的初步研究  

Characteristics of cancer stem cells in human colon cancer cell lines

作　　者：陈克力[1] 江恒[1] 陈建芳[1] 裴莉[1] 潘凤[1] 梁后杰[1] 

机构地区：[1]第三军医大学西南医院肿瘤科,重庆400038

出　　处：《中华肿瘤防治杂志》2009年第21期1646-1650,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：重庆市自然科学基金(CSTC2009BB5328)

摘　　要：目的:本研究对8种人结肠癌细胞系的干细胞特性进行初步检测,以寻找适合分选结肠癌干细胞的细胞系。方法:利用流式细胞术,RT-PCR检测8种结肠癌细胞中CD133的表达情况;利用条件培养观察8种细胞系的肿瘤细胞在无血清培养基中的生物学特性;利用裸鼠成瘤模型检测成瘤能力和转移能力。结果:CD133在8种细胞系中的表达有显著差异,最高的HCT116细胞系中CD133+细胞含量为(91.4±5.0)%;各种细胞系细胞均能在无血清培养基中增殖,但只有HCT116细胞能形成典型的"神经球样结构"且具有较高的成瘤能力和转移能力。同等细胞浓度下移植瘤体积HCT116(1.06±0.13)cm3,LOVO(0.22±0.09)cm3,P=0.01,差异具有统计学意义。结论:相对于其他7种细胞系,HCT116细胞具有多种肿瘤干细胞的生物学特性,更适合于结肠癌干细胞的研究。OBJECTIVE：To investigate the characteristics of colon cancer stem cells in colon cancer cell lines and look for suitable cell lines applying to isolate colon cancer stem cells.METHODS：The expressions of CD133 in eight cell lines were investigated by flow cytometry and RT-PCR.The different cancer cells were cultured in serum-free medium for the study of their Biological characteristics.The tumor model in nude mice was used to evaluate the tumorigenicity and metastasis of different cancer cells.RESULTS：The expressions of CD133 in eight cell lines were significantly different and the highest HCT116 cell line contained（91.4±5.0）% CD133＋ cells.Eight types of cancer cells could proliferate in serum-free medium,but only HCT116 cells could form the typical ＂neurosphere＂ structure and had high tumorigenicity.At the same cell density,the xenografts formed by HCT116 cells（1.06±0.13）cm3 were statistically bigger than that of LOVO cells（0.22±0.09）cm3,P=0.01.CONCLUSION：Compared with other seven cell lines,HCT116 possesses the main defining characteristics of colon cancer stem cells,so it could be a suitable cell line for researches of colon cancer stem cells.

关 键 词：结肠肿瘤 细胞系 肿瘤 肿瘤干细胞 细胞培养 抗原 CD 

分 类 号：R735.35[医药卫生—肿瘤]