兔软骨细胞诱导人脐血间充质干细胞成软骨的可行性  

Feasibility of differentiating human umbilical cord blood stem cells into chondrocytes induced by rabbit chondrocytes

作　　者：李丽艳[1] 杜江[1] 黄金中[1] 

机构地区：[1]南方医科大学耳鼻喉科,广东省广州市510515

出　　处：《中国组织工程研究与临床康复》2009年第49期9713-9716,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：广东省自然科学基金资助项目(990418)~~

摘　　要：背景:在众多已被人们认识的可以治疗软骨缺损的细胞如胚胎干细胞、骨髓间充质干细胞等被认为最具有运用前景。但由于来源有限,取材困难,涉及伦理及技术等方面的问题,严重地限制了其实用性。因此,寻找新的实用的细胞来源有着重要意义。目的:观察人脐血干细胞在体外诱导分化为软骨细胞的可行性。方法:由足月分娩的脐静脉穿刺抽取获得间充质干细胞,并用BrdU标记,用Transwell技术共培养兔软骨细胞诱导人脐血干细胞,免疫组织化学鉴定诱导后的细胞。结果与结论:经兔软骨细胞诱导后的人脐带血间充质干细胞分化为软骨细胞,胞质同正常软骨细胞一样被染成紫色,BrdU在细胞核表现为黄色。提示人脐血干细胞在体外可以分化成软骨细胞。BACKGROUND：Embryonic stem cells or bone marrow mesenchymal stem cells possess potential to treat cartilage defects,but the practicability is restricted by many factors.Therefore,it is significant to explore a new source of cells.OBJECTIVE：To investigate the feasibility of differentiating human umbilical cord blood stem cells into chondrocytes.METHODS：Human mesenchymal stem cells was obtained from term delivered umbilical vein,labeled by BrdU,and co-cultured with rabbit chondrocytes using Transwell method.The harvested cells were identified by immunocytochemistrical analysis.RESULTS AND CONCLUSION：The human umbilical cord blood stem cells were differentiated into chondrocytes under induction of rabbit chondrocytes,cytoplasm of which were stained into purple,and cellular nucleus was yellow stained with BrdU.The results revealed that human umbilical cord blood stem cells can in vitro differentiate into chondrocytes.

关 键 词：脐带血 间充质干细胞 软骨细胞 BRDU 

分 类 号：R394.2[医药卫生—医学遗传学]