大鼠自体骨髓间充质干细胞移植诱导缺血肢体血管生成:血浆及缺血组织中单核细胞趋化蛋白1变化的意义(英文)  

Autologous mesenchymal stem cell transplantation induces angiogenesis in rat ischemic limbs Significance of monocyte chemoattractant protein-1 changes in plasma and ischemic tissues

作　　者：霍鑫[1] 张强[1] 

机构地区：[1]中国医科大学附属第一医院干诊外科,辽宁省沈阳市110001

出　　处：《中国组织工程研究与临床康复》2009年第49期9771-9774,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:观察大鼠自体骨髓间充质干细胞移植诱导缺血肢体血管生成过程中,缺血组织单核细胞趋化蛋白1的变化。方法:雄性SD大鼠20只,随机分为模型组、细胞移植组,10只/组。抽取大鼠股骨骨髓,percoll密度梯度法分离培养骨髓间充质干细胞,取传至第3或4代的细胞用于移植。两组大鼠均建立急性双下肢缺血模型,造模后2h,细胞移植组于双下肢缺血部位缓慢注射1×1011L-1骨髓间充质干细胞,模型组同法注射等量磷酸盐缓冲液。血管造影和组织学毛细血管密度检查侧支血管形成情况;免疫组化法检查CD68+的巨噬细胞浸润情况;ELISA法检测血浆及缺血组织单核细胞趋化蛋白1蛋白的表达;RT-PCR法检测缺血组织单核细胞趋化蛋白1mRNA的表达。结果:移植后28d,细胞移植组缺血下肢可见明显侧支血管形成,且免疫组化显示CD68+巨噬细胞的浸润少于模型组;与模型组比较,细胞移植组血浆、缺血组织单核细胞趋化蛋白1的蛋白浓度和mRNA表达水平均明显降低(P<0.05)。结论:骨髓间充质干细胞移植后,单核细胞趋化蛋白1可能在缺血诱导血管生成的过程中起重要作用,提示其可成为一个调节骨髓间充质干细胞移植炎性血管生成的靶分子。OBJECTIVE：To observe monocyte chemoattractant protein 1 （MCP-1） changes in ischemic tissue during the process of angiogenesis induction in ischemic limbs by autologous mesenchymal stem cell （MSC） transplantation.METHODS：Twenty male Sprague-Dawley rats were randomized to 2 groups （n=10）：model and MSC transplantation.Femoral and tibial bone marrow was taken to isolate and culture MSCs by percoll density gradient method.Cells of the 3rd or 4th passage were used for transplantation.Severe bilateral hind limb ischemia was surgically created in each group rats.Two hours after model establishment,MSCs （1×10^11/L） were infused into the ischemic region of rats from the MSC transplantation group,and the model group received the same amount of phosphate buffered saline.Collateral artery formation was determined by angiographic analysis and histological assessment.CD68＋ macrophage infiltration was examined by immunohistochemistry.MCP-1 protein expression in the plasma and ischemic tissue was detected by enzyme-linked immunosorbent assay.MCP-1 mRNA expression in ischemic tissue was detected by reverse transcription-polymerase chain reaction.RESULTS：At postoperative 28 days,treatment with MSC transplantation lead to collateral vessel formation,and immunohistochemistry demonstrated that CD68＋ macrophage infiltration was lower compared with the model group.MCP-1 protein and mRNA expression in the plasma and ischemia tissue was significantly lower in the MSC transplantation group than in the model group （P 〈0.05）.CONCLUSION：Following MSC transplantation,MCP-1 may play an important role in ischemia-induced angiogenesis.This indicates that MCP-1 would become one possible target molecule for modulating inflammatory angiogenesis by MSC Transplantation.

关 键 词：骨髓间充质干细胞 移植 血管生成 缺血 单核细胞趋化蛋白1 

分 类 号：R394.2[医药卫生—医学遗传学]